Liquid chromatography- tandem mass spectrometry characterization of five new leucinostatins produced by Paecilomyces lilacinus CG-189

Autor(es)

Canovas Martinez, Ana Flavia ^[1] ; Beraldo Moraes, Luiz Alberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Chem, Lab Mass Spectrometry Appl Nat Prod, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ANTIBIOTICS; v. 68, n. 3, p. 178-184, MAR 2015.
• Citações Web of Science: 3

Resumo

The fungus Paecilomyces lilacinus produces leucinostatins-peptaibiotics that exert a range of biological activities including antimalarial, antiviral, antitumor and phytotoxicity. In this paper, we developed an analytical method employing LC-MS/MS in the precursor ion and product ion scan modes to elucidate five new leucinostatins. Direct Infusion (DI-MS) helped to identify the most abundant leucinostatins: F, D B2, S, A and K. MS/MS analysis using a triple quadrupole operating at different scan modes is a versatile tool to study natural products, especially peptaibiotics. Although DI-MS full-scan analysis is rapid and sensitive, it cannot distinguish between peptide isomers. On the other hand, LC-MS/MS operated in the precursor ion and product ion modes is time consuming, but allows identifying the structure of isomers or isobar in crude extracts. (AU)

• Processo FAPESP: 09/54094-0 - EMU: aquisição de um espectrômetro de massas de estágios múltiplos para estruturação do Laboratório / Facilities do Departamento de Química da FFCLRP-USP
• Beneficiário: Luiz Alberto Beraldo de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários