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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Liquid chromatography- tandem mass spectrometry characterization of five new leucinostatins produced by Paecilomyces lilacinus CG-189

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Author(s):
Canovas Martinez, Ana Flavia [1] ; Beraldo Moraes, Luiz Alberto [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Chem, Lab Mass Spectrometry Appl Nat Prod, BR-14040901 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF ANTIBIOTICS; v. 68, n. 3, p. 178-184, MAR 2015.
Web of Science Citations: 3
Abstract

The fungus Paecilomyces lilacinus produces leucinostatins-peptaibiotics that exert a range of biological activities including antimalarial, antiviral, antitumor and phytotoxicity. In this paper, we developed an analytical method employing LC-MS/MS in the precursor ion and product ion scan modes to elucidate five new leucinostatins. Direct Infusion (DI-MS) helped to identify the most abundant leucinostatins: F, D B2, S, A and K. MS/MS analysis using a triple quadrupole operating at different scan modes is a versatile tool to study natural products, especially peptaibiotics. Although DI-MS full-scan analysis is rapid and sensitive, it cannot distinguish between peptide isomers. On the other hand, LC-MS/MS operated in the precursor ion and product ion modes is time consuming, but allows identifying the structure of isomers or isobar in crude extracts. (AU)

FAPESP's process: 09/54094-0 - Acquisition of a multi-stage mass spectrometer for a Laboratory / Facility in the Chemistry Department of the University of São Paulo Ribeirão Preto School of Philosophy, Science and Literature
Grantee:Luiz Alberto Beraldo de Moraes
Support Opportunities: Multi-user Equipment Program