The toxicology of New Psychoactive Substances (NSP): epidemiology of consumption by oral fluid samples and characterization of their in vitro metabolism

• Grant number: 18/11849-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2018
• End date: September 05, 2022
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: José Luiz da Costa
• Grantee: Kelly Francisco da Cunha
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications, AP.TEM
• Associated scholarship(s): 19/23954-6 - Analysis of new psychoactive substances (NPs) in oral fluid samples by high-resolution mass spectrometry and in vitro metabolism study, BE.EP.DR

Abstract

The number of new psychoactive substances (NPS) has grown exponentially in recent years. Due to their high capacity to generate effects in low doses, the number of intoxications has grown along with the consumption. While some NPS do not fall under the current anti-drug legislation, this substance gains easy access to users mainly through the internet, making their control more difficult. Despite the international surveys on seizures and NPS consumption, in Brazil these data are scarce, restricting themselves to cases of intoxication reported in the literature, and these numbers are probably underestimated. Objectives: the aim of this work is to generate the first epidemiological data on NPS consumption in São Paulo state, by collection and analysis of oral fluid samples of participant of electronic music parties. From these data, we will also analyze the in vitro metabolism of the most recurrent NPS that do not yet present this type of study. Material and method: The collection will be voluntary and anonymous, where the volunteer will answer a brief questionnaire and, in return, receive a card with number and specific passwords of his sample to access the result of the analysis in a site developed for this purpose. Samples will be extracted by Microextraction by Packed Sorbent (MEPS) and liquid-liquid extraction and analyzed by liquid chromatography-tandem mass spectrometry. In vitro metabolism of the most recurrent NPS will be analyzed with Human Liver Microsome (HLM) and possible metabolites will be identified by liquid chromatography-high resolution mass spectrometry. Expected results: It is expected to generate the first data on the consumption of this new and important group of drugs of abuse in the electronic music festival environment in order to establish the main substances in circulation. From the studies of in vitro metabolism, it is expected to predict the toxicokinetics of the most incident substances in this context, as well as structural elucidation of their main metabolites. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA CUNHA, KELLY FRANCISCO; RODRIGUES, LEONARDO COSTALONGA; HUESTIS, MARILYN A.; COSTA, JOSE LUIZ. Miniaturized extraction method for analysis of synthetic opioids in urine by microextraction with packed sorbent and liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, v. 1624, AUG 2 2020. (17/02147-0, 18/11849-0, 17/24515-0, 18/00432-1)

DA CUNHA, KELLY FRANCISCO; OLIVEIRA, KARINA DINIZ; HUESTIS, MARILYN A.; COSTA, JOSE LUIZ. Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC-MS-MS. JOURNAL OF ANALYTICAL TOXICOLOGY, v. 44, n. 7, p. 697-707, SEP 2020. (17/02147-0, 18/11849-0, 18/00432-1)

FURIOZO ARANTES, ANA CAROLINA; DA CUNHA, KELLY FRANCISCO; CARDOSO, MARILIA SANTORO; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. Development and validation of quantitative analytical method for 50 drugs of antidepressants, benzodiazepines and opioids in oral fluid samples by liquid chromatography-tandem mass spectrometry. Forensic Toxicology, v. 39, n. 1, DEC 2020. (18/00432-1, 18/11849-0)

DA CUNHA, KELLY FRANCISCO; OLIVEIRA, KARINA DINIZ; CARDOSO, MARILIA SANTORO; FURIOZO ARANTES, ANA CAROLINA; PIRAS COSER, PEDRO HENRIQUE; LIMA, LUCAS DE NORONHA; SAMPAIO MALUF, ANA CRISTHINA; DE CASTRO COMIS, MARIA ANGELICA; HUESTIS, MARILYN A.; COSTA, JOSE LUIZ. Prevalence of new psychoactive substances (NPS) in Brazil based on oral fluid analysis of samples collected at electronic music festivals and parties. DRUG AND ALCOHOL DEPENDENCE, v. 227, OCT 1 2021. (17/02147-0, 18/11849-0, 18/00432-1)

DA CUNHA, KELLY FRANCISCO; MAGALHAES KAHL, JULIA MARTINELLI; FIORENTIN, TAIS REGINA; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. High-sensitivity method for the determination of LSD and 2-oxo-3-hydroxy-LSD in oral fluid by liquid chromatography-tandem mass spectrometry. Forensic Toxicology, v. 40, n. 2, p. 10-pg., 2022-04-12. (20/07470-6, 18/11849-0, 20/10809-5, 18/00432-1)

DA CUNHA, KELLY FRANCISCO; LANARO, RAFAEL; MARTINS, ALINE FRANCO; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. Use of injection-port derivatization for the analysis of cocaine and its metabolites in urine by gas chromatography-tandem mass spectrometry. Forensic Toxicology, v. 39, n. 1, p. 8-pg., 2020-08-01. (17/02147-0, 18/11849-0, 18/00432-1)

DA CUNHA, KELLY FRANCISCO; LANARO, RAFAEL; MARTINS, ALINE FRANCO; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. Use of injection-port derivatization for the analysis of cocaine and its metabolites in urine by gas chromatography-tandem mass spectrometry. Forensic Toxicology, AUG 2020. (17/02147-0, 18/00432-1, 18/11849-0)

PEREIRA, JIAN F. S.; MELO, LARISSA M. A.; ARANTES, LUCIANO C.; BORGES, PEDRO H. S.; FARIA, LUCAS, V; DA CUNHA, KELLY F.; SOUZA, KARLA A. O.; SOTE, WILLIAM O.; COSTA, JOSE LUIZ; NOSSOL, EDSON; et al. Oxygen plasma-treated graphite sheet electrodes: A sensitive and disposable sensor for methamphetamines. Electrochimica Acta, v. 467, p. 13-pg., 2023-09-02. (18/11849-0, 18/00432-1)

DE MORAIS, DAMILA RODRIGUES; DA CUNHA, KELLY FRANCISCO; RODRIGUES, TAIS BETONI; LANARO, RAFAEL; BARBOSA, LILIAN DE MELO; ZACCA, JORGE JARDIM; EBERLIN, MARCOS NOGUEIRA; COSTA, JOSE LUIZ. Triple quadrupole-mass spectrometry protocols for the analysis of NBOMes and NBOHs in blotter papers. Forensic Science International, v. 309, APR 2020. (18/00432-1, 16/23157-0, 18/11849-0)

DA CUNHA, KELLY FRANCISCO; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. Green analytical toxicology method for determination of synthetic cathinones in oral fluid samples by microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry. Forensic Toxicology, v. N/A, p. 13-pg., 2023-07-28. (17/02147-0, 20/10809-5, 18/11849-0, 18/00432-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CUNHA, Kelly Francisco da. The toxicology of New Psychoactive Substances (NSP): epidemiology of consumption through the analysis of oral fluid samples and characterization of their in vitro metabolism. 2022. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.