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The toxicology of New Psychoactive Substances (NSP): epidemiology of consumption by oral fluid samples and characterization of their in vitro metabolism

Grant number: 18/11849-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2018
Effective date (End): September 05, 2022
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal researcher:José Luiz da Costa
Grantee:Kelly Francisco da Cunha
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications, AP.TEM
Associated scholarship(s):19/23954-6 - Analysis of new psychoactive substances (NPs) in oral fluid samples by high-resolution mass spectrometry and in vitro metabolism study, BE.EP.DR


The number of new psychoactive substances (NPS) has grown exponentially in recent years. Due to their high capacity to generate effects in low doses, the number of intoxications has grown along with the consumption. While some NPS do not fall under the current anti-drug legislation, this substance gains easy access to users mainly through the internet, making their control more difficult. Despite the international surveys on seizures and NPS consumption, in Brazil these data are scarce, restricting themselves to cases of intoxication reported in the literature, and these numbers are probably underestimated. Objectives: the aim of this work is to generate the first epidemiological data on NPS consumption in São Paulo state, by collection and analysis of oral fluid samples of participant of electronic music parties. From these data, we will also analyze the in vitro metabolism of the most recurrent NPS that do not yet present this type of study. Material and method: The collection will be voluntary and anonymous, where the volunteer will answer a brief questionnaire and, in return, receive a card with number and specific passwords of his sample to access the result of the analysis in a site developed for this purpose. Samples will be extracted by Microextraction by Packed Sorbent (MEPS) and liquid-liquid extraction and analyzed by liquid chromatography-tandem mass spectrometry. In vitro metabolism of the most recurrent NPS will be analyzed with Human Liver Microsome (HLM) and possible metabolites will be identified by liquid chromatography-high resolution mass spectrometry. Expected results: It is expected to generate the first data on the consumption of this new and important group of drugs of abuse in the electronic music festival environment in order to establish the main substances in circulation. From the studies of in vitro metabolism, it is expected to predict the toxicokinetics of the most incident substances in this context, as well as structural elucidation of their main metabolites. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FURIOZO ARANTES, ANA CAROLINA; DA CUNHA, KELLY FRANCISCO; CARDOSO, MARILIA SANTORO; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. Development and validation of quantitative analytical method for 50 drugs of antidepressants, benzodiazepines and opioids in oral fluid samples by liquid chromatography-tandem mass spectrometry. Forensic Toxicology, v. 39, n. 1, . (18/00432-1, 18/11849-0)
DA CUNHA, KELLY FRANCISCO; OLIVEIRA, KARINA DINIZ; CARDOSO, MARILIA SANTORO; FURIOZO ARANTES, ANA CAROLINA; PIRAS COSER, PEDRO HENRIQUE; LIMA, LUCAS DE NORONHA; SAMPAIO MALUF, ANA CRISTHINA; DE CASTRO COMIS, MARIA ANGELICA; HUESTIS, MARILYN A.; COSTA, JOSE LUIZ. Prevalence of new psychoactive substances (NPS) in Brazil based on oral fluid analysis of samples collected at electronic music festivals and parties. DRUG AND ALCOHOL DEPENDENCE, v. 227, . (17/02147-0, 18/11849-0, 18/00432-1)
DA CUNHA, KELLY FRANCISCO; RODRIGUES, LEONARDO COSTALONGA; HUESTIS, MARILYN A.; COSTA, JOSE LUIZ. Miniaturized extraction method for analysis of synthetic opioids in urine by microextraction with packed sorbent and liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, v. 1624, . (18/00432-1, 17/02147-0, 18/11849-0, 17/24515-0)
DA CUNHA, KELLY FRANCISCO; OLIVEIRA, KARINA DINIZ; HUESTIS, MARILYN A.; COSTA, JOSE LUIZ. Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC-MS-MS. JOURNAL OF ANALYTICAL TOXICOLOGY, v. 44, n. 7, p. 697-707, . (17/02147-0, 18/11849-0, 18/00432-1)
DE MORAIS, DAMILA RODRIGUES; DA CUNHA, KELLY FRANCISCO; RODRIGUES, TAIS BETONI; LANARO, RAFAEL; BARBOSA, LILIAN DE MELO; ZACCA, JORGE JARDIM; EBERLIN, MARCOS NOGUEIRA; COSTA, JOSE LUIZ. Triple quadrupole-mass spectrometry protocols for the analysis of NBOMes and NBOHs in blotter papers. Forensic Science International, v. 309, . (18/00432-1, 16/23157-0, 18/11849-0)
DA CUNHA, KELLY FRANCISCO; LANARO, RAFAEL; MARTINS, ALINE FRANCO; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. Use of injection-port derivatization for the analysis of cocaine and its metabolites in urine by gas chromatography-tandem mass spectrometry. Forensic Toxicology, . (17/02147-0, 18/00432-1, 18/11849-0)

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