Gene expression investigation, related to the identified proteins in the previous salivary proteome study, in individuals with Type 2 Diabetes Mellitus, Dyslipidemia and Chronic Periodontal Disease

• Grant number: 17/10317-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2017
• End date: December 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Sâmia Cruz Tfaile Corbi
• Grantee: Maria Letícia Verdi Emílio
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The aim of this study is to investigate the gene expression of PIGR (Polymeric Immunoglobulin Receptor) and LCP1 (Plastin-2) genes, which are related to the salivary proteins identified in the previous study about Salivary Proteome in patients affected by type 2 diabetes mellitus - DM2 (metabolically compensated and uncompensated), dyslipidemia and chronic periodontal disease. The development of the present study aims to complement the recently concluded Post-Doctoral Fellow of the candidate for orientation of this project, supported by FAPESP (Grant BEPE - Canada: 2015 / 08678-1) and currently performing postdoctoral studies in Brazil under Supervision of Profa. Dra. Raquel M. Scarel Caminaga (Post-Doctoral Grant - Brazil: 2014 / 16148-0), in which evaluated the Salivary and Plasma Proteome of individuals simultaneously presenting or not these three pathologies, using identification, characterization and quantification of the proteins by SDS-PAGE (12%) followed by Reverse Phase Liquid Chromatography and Tandem Liquid Chromatography in Mass Spectrometry (LC-ESI-MS / MS). Five groups of patients (with 30 patients each) were investigated: Group 1 - Individuals with DM2, metabolically uncompensated (HbA1c e 8.0%), dyslipidemia and PD; Group 2- Individuals with DM2, metabolically compensated (HbA1c <8.0%), with dyslipidemia and PD; Group 3 - Individuals without DM2, with dyslipidemia and with PD; Group 4 - Individuals without DM2, without dyslipidemia and with PD; Group 5- Individuals without DM2, without dyslipidemia and without PD. Considering the Salivary Proteome of these individuals, the final result was the identification and characterization of some proteins, among them the Polymeric Immunoglobulin Receptor and Plastin-2 proteins, which are related to immune response and cellular organization. In addition, the relative quantification of abundance results showed that these two salivary proteins are in greater abundance in the diseased individuals groups compared to the group of healthy individuals (Control Group) - results are being finalized for publication in international journals. All patients, from the five groups, were submitted to complete periodontal examination, physical examination and biochemical evaluation of glycemic and lipid profiles. The blood of all patients was collected, and the total RNA was extracted. In this present study the cDNA of all samples of the 5 different groups of individuals will be made to investigate the gene expression of the PIGR and LCP1 genes by Real Time PCR (qPCR) using the TaqMan system. It is important to carry out this study, because the gene expression of these genes related to the proteins identified as differentially abundant in each condition can be investigated as new therapeutic targets, besides being useful for the diagnosis and clinical follow-up of the patients affected by these pathologies. In addition, it is also expected to contribute to the elucidation of the molecular mechanisms involved in these diseases, allowing us better understanding the possible interrelationship of several important genes and their influence on the glycemic, lipid and periodontal profile of the patients with the mentioned pathologies. (AU)