Expression of interleukin 7 and Profilin 1 genes in patients with diabetes mellitus type 2, dyslipidemia and periodontal disease

• Grant number: 18/26338-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2019
• End date: March 31, 2021
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Raquel Mantuaneli Scarel Caminaga
• Grantee: Karina da Silva Ferreira
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Diabetes mellitus type 2 (DM2) and Dyslipidemia systemic diseases are involved in the pathogenesis of periodontal disease (PD) and may alter the immune-inflammatory response of the host. The objective of this study is to investigate the expression of genes involved in the inflammatory process interleukin 7 (IL7) and Profilin 1 (PFN1) in patients with DM2 (compensated and decompensated), Dyslipidemia and DP. In this way, it will be possible to complement the results of an earlier study conducted by our research group with the support of FAPESP (Doctoral Grant FAPESP 2009 / 16233-9, and Research Grant FAPESP 2010 / 10882-2), which has the Transcriptome PBMC cells blood mononuclear cells) from the same individuals by means of microarray. It was verified the expression of more than 54,000 genes (transcripts) and bioinformatics analysis it was possible to explore potential pathways of gene interactions (metabolic pathways) in which the presence of the IL7 and PFN1 genes modulated by the presence of these three pathological conditions. Yet was not seen in the literature any study investigating the expression of these genes in patients who manifest simultaneously the three diseases mentioned. Prior to this research project, our team investigated five groups of patients (30 patients each): DMdDisDP (decompensated type 2 diabetes, dyslipidemia and periodontal disease), DMcDisDP (compensated diabetes, dyslipidemia and periodontal disease), DisDP (only dyslipidemia and periodontal disease), cDP (only periodontal disease) and control (without any of the three diseases). All patients underwent a complete periodontal examination, physical examination and biochemical evaluation of glycemic and lipid profiles. From each patient, blood was collected, and the RNA extracted. In this present IC project will be made cDNA samples for investigation of the expression of IL7 and PFN1 gene by Real-Time PCR (qPCR) using the TaqMan system. It is intended to verify if the results with RT-qPCR to be obtained validate those previously observed by microarray, besides investigating the potential interrelationship of these genes and their influence on glucose, lipid profile and periodontal patients with the aforementioned conditions.