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Transcriptome and salivary and plasma proteome investigations in individuals with type 2 diabetes mellitus, dyslipidemia and chronic periodontal disease

Grant number: 14/16148-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2015
Effective date (End): December 31, 2018
Field of knowledge:Health Sciences - Dentistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Raquel Mantuaneli Scarel Caminaga
Grantee:Sâmia Cruz Tfaile Corbi
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated scholarship(s):15/08678-1 - Salivary and plasma proteome investigations in individuals with type 2 diabetes mellitus, dyslipidemia and chronic periodontal disease, BE.EP.PD

Abstract

The aim of this project is to conduct further analysis of the transcriptome (study 1) and investigate the salivary and plasma proteome (study 2) related to individuals affected by type 2 Diabetes Mellitus (DM2, metabolically compensated/decompensated), Dyslipidemia and Chronic Periodontal Disease (DP). The development of the study 1 aims to complement the recently completed PhD study from the requestor (FAPESP 2009/16233-9 and 2010/10882-2) in which five groups of patients were examined (30 patients each): Group 1 (= 30) - Individuals with DM2, metabolically decompensated (HbA1c e 8.0%) with dyslipidemia and PD; Group2 (n=30) -Individuals with DM2, metabolically compensated (HbA1c < 8.0%) with dyslipidemia and PD; Group3 (n=30) - Individuals without DM2, with dyslipidemia and with PD; Group4 (n=30) - Individuals without DM2, without dyslipidemia and with DP; Group5 (n =30) - Individuals without DM2, without dyslipidemia and without PD. In the PhD study the microarray data were analyzed in the following ways: Group 1 + Group 2 vs Group 3; Group 1 vs Group 2; Group 1 vs Group 3, Group 2 vs Group 3; Group 3 vs Group 4; Group 4 vs Group 5. To complement the results of the transcriptome, this study 1 propose to perform analysis of Bioinformatics and Biostatistics similarly to the comparisons above, but always considering as control group 5; as follows: Group 1 + Group 2 vs Group 5; Group 1 vs Group 5; Group 5 vs Group 2; Group 3 vs Group 5. Will be selected in the aforementioned comparisons of the groups, 2-3 differentially expressed genes (down/overexpressed) and may play a role in the pathophysiology of the alterations analyzed for validation by RT-qPCR (Reverse Transcriptase-followed by Polymerase Chain Reaction in quantitative real time). In the study 2 will be investigated the salivary and plasma proteome of the same groups of the individuals of the study 1 for identification, characterization and quantification of more/less abundant proteins in saliva and plasma. The SDS-PAGE gels will be prepared in polyacrylamide gradient between 4 and 20% and also will be made the native PAGE gels for separation and quantification of proteins. Then, will be used the Reverse Phase Liquid Chromatography and Liquid Chromatography Tandem Mass Spectrometry. Will be selected a protein with greater abundance and another with lower abundance in every Group 1, Group 2, Group 3, Group 4 in comparison with Group 5 (considered the control group) for validation by ELISA or Western blotting. It is important to undertake this present study, because the genes and/or proteins differentially expressed in each condition, after validated, may be investigated as a new therapeutic targets, and are useful for the diagnosis and monitoring of patients affected by these diseases. Furthermore, it is also expected to contribute to the elucidation of the molecular mechanisms involved in these diseases. (AU)

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