Investigation of biofilm production and icaA and icaD genes in Staphylococcus aureus isolated from heifers and cows with mastitis

Autor(es)

Castelani, Livia ^{[1, 2]} ; Pilon, Lucas Eduardo ^{[1, 3]} ; Martins, Thamires ^[1] ; Pozzi, Claudia Rodrigues ^[1] ; Pozzi Arcaro, Juliana Rodrigues ^[1]

Afiliação do(s) autor(es)

^[1] IZ Inst Zootecnia, Sao Paulo - Brazil

^[2] Univ Sao Paulo, ICB USP Inst Biomed Sci, Dept Microbiol, BR-05508900 Butanta Sao Paulo, SP - Brazil

^[3] UNESP Univ Estadual Paulista Julio de Mesquita Fi, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANIMAL SCIENCE JOURNAL; v. 86, n. 3, p. 340-344, MAR 2015.
• Citações Web of Science: 8

Resumo

Biofilm formation and antimicrobial resistance of Staphylococcus aureus are important virulence factors in cases of mastitis in dairy cows. However, few studies have investigated mastitis strains isolated from heifers. Within this context, the objective of the present study was to investigate biofilm formation on Congo red agar, the presence of the icaA and icaD genes by polymerase chain reaction (PCR), and the percentage of in vitro antimicrobial resistance of 110 S.aureus isolates from mammary gland secretions of heifers and cows with mastitis. PCR detected the icaA and icaD genes in 98% and 100% of isolates, respectively. However, only 55.5% of all isolates produced a biofilm on Congo red agar. Antimicrobial susceptibility testing revealed that 47.0% of isolates from heifers and 70.4% of isolates from cows were resistant to at least one of the antimicrobial agents tested. Resistance to penicillin and/or ampicillin was the most frequent (44.5%). These results indicate the need to implement prophylactic and control measures of mastitis for heifers. Heifers and cows can carry resistant strains with the capacity of biofilm production, a fact representing a threat to public health and animal well-being and generating losses to dairy farmers. (AU)

• Processo FAPESP: 10/02038-7 - Genótipos de virulência, perfil de resistência e tipagem molecular de cepas de Staphylococcus aureus associados à mastite de animais provenientes dos estados do Paraná e de São Paulo
• Beneficiário: Lívia Castelani
• Modalidade de apoio: Bolsas no Brasil - Mestrado