Optical Characterization of Normal, Benign, and Malignant Thyroid Tissue: A Pilot Study

Autor(es)

Brandao, M. P. ^[1] ; Iwakura, R. ^[2] ; Basilio, F. S. ^[2] ; Haleplian, K. ^[1] ; Ito, A. S. ^[1] ; Conti de Freitas, L. C. ^[2] ; Bachmann, L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Fis, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Oftalmo Otorrino & Cirurgia Cabeca & Pescoco, Fac Med Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Fluorescence; v. 25, n. 2, p. 465-471, MAR 2015.
• Citações Web of Science: 4

Resumo

Fine-needle aspiration cytology is the standard technique to diagnose thyroid pathologies. However, this method has a high percentage of inconclusive and false-negative results for benign and malignant lesions. Hence, it is important to search for a new method to assist medical evaluation during these surgical procedures. The use of time-resolved fluorescence techniques to detect biochemical composition and tissue structure alterations could help to develop a portable, minimally invasive, and non-destructive method to assist medical evaluation. In this study, we investigated 17 human thyroid samples by absorbance, fluorescence, excitation, and time-resolved fluorescence measurements. This initial investigation has demonstrated that thyroid fluorescence originates from many endogenous fluorophores and culminates in several bands. The fluorescence lifetimes of benign and malignant lesions were significantly different, as attested by analysis of variance using Tukey test with individual confidence level of 98.06 %. Our results suggest that fluorescence lifetimes of benign and malignant lesions can potentially assist diagnosis. After further investigations, fluorescence methods could become a tool for the surgeon to identify differences between normal and pathological thyroid tissues. (AU)

• Processo FAPESP: 12/02460-6 - Espectroscopia vibracional de tecidos biológicos: diagnóstico de lesões cancerosas e caracterização química de tecidos dentais irradiados com lasers
• Beneficiário: Luciano Bachmann
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/07960-4 - Radiometria ótica de lasers, LEDs e lâmpadas emissoras de radiação ultravioleta
• Beneficiário: Luciano Bachmann
• Modalidade de apoio: Auxílio à Pesquisa - Regular