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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Optical Characterization of Normal, Benign, and Malignant Thyroid Tissue: A Pilot Study

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Author(s):
Brandao, M. P. [1] ; Iwakura, R. [2] ; Basilio, F. S. [2] ; Haleplian, K. [1] ; Ito, A. S. [1] ; Conti de Freitas, L. C. [2] ; Bachmann, L. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Fis, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Oftalmo Otorrino & Cirurgia Cabeca & Pescoco, Fac Med Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Fluorescence; v. 25, n. 2, p. 465-471, MAR 2015.
Web of Science Citations: 4
Abstract

Fine-needle aspiration cytology is the standard technique to diagnose thyroid pathologies. However, this method has a high percentage of inconclusive and false-negative results for benign and malignant lesions. Hence, it is important to search for a new method to assist medical evaluation during these surgical procedures. The use of time-resolved fluorescence techniques to detect biochemical composition and tissue structure alterations could help to develop a portable, minimally invasive, and non-destructive method to assist medical evaluation. In this study, we investigated 17 human thyroid samples by absorbance, fluorescence, excitation, and time-resolved fluorescence measurements. This initial investigation has demonstrated that thyroid fluorescence originates from many endogenous fluorophores and culminates in several bands. The fluorescence lifetimes of benign and malignant lesions were significantly different, as attested by analysis of variance using Tukey test with individual confidence level of 98.06 %. Our results suggest that fluorescence lifetimes of benign and malignant lesions can potentially assist diagnosis. After further investigations, fluorescence methods could become a tool for the surgeon to identify differences between normal and pathological thyroid tissues. (AU)

FAPESP's process: 12/02460-6 - Vibrational spectroscopy applied to biological tissues: cancer diagnosis and chemical characterization of laser irradiated dental tissues
Grantee:Luciano Bachmann
Support Opportunities: Regular Research Grants
FAPESP's process: 11/07960-4 - Optical radiometry of lasers, LEDs and ultraviolet lamps
Grantee:Luciano Bachmann
Support Opportunities: Regular Research Grants