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Nuclear morphometry and chromatin textural characteristics of thyroid follicular lesions

Grant number: 14/10028-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2014
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Principal researcher:Gláucia Maria Ferreira da Silva Mazeto
Grantee:Bárbara Parente Coelho
Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Context: Papillary carcinoma (PC) and follicular (FC) represent the most common differentiated thyroid carcinoma (DTC). There are several histological subtypes of CP, with the classic (CPC) the most frequent, followed by follicular variant (FVPC). For diagnosis of DTC, the most widely used method is the fine needle aspiration (FNA) with cytological analysis of the aspirate. Recently, the classification of Bethesda standardized the cytologic diagnosis of thyroid lesions in six classes: nondiagnostic/unsatisfactory, benign, atypia of undetermined significance/follicular lesion of undetermined significance, follicular neoplasm /suspicious for follicular neoplasm, suspicious for malignancy and malignant (classes I to VI, respectively). However, the diagnosis of classes III, IV and V, associated with increased risk of malignancy, are not totally assertive. In this context, the evaluation of nuclear characteristics of follicular cells with computerized image analysis could be useful in differentiating between benign and malignant lesions. Objectives: To compare, by computerized image analysis, histological material of follicular adenomas, follicular carcinoma, follicular variant of papillary carcinoma and normal thyroid tissue in relation to characteristics of the cell nuclei. Methodology: will be review reports histological diagnoses of patients undergoing thyroidectomy between 12/2003-12/2013, in the Clinics Hospital-Botucatu Medical School. Will be selected slides with histological material of follicular adenoma (FA), CF and FVPC. The cell nuclei will be photographed through an optical microscope coupled to digital camera and the images evaluated using the computer program ImageJ. Nuclei will be compared for size, shape, overlapping, crowding, clearing, nuclear clefts, irregularity of nuclear membrane and chromatin texture.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BÁRBARA PARENTE COELHO; FLÁVIA DE OLIVEIRA VALENTIM; HÉLIO AMANTE MIOT; DANILO TAKESHI ABE JAUNE; CAROLINE YUKI HAYASHI; CRISTIANO CLAUDINO DE OLIVEIRA; MARIÂNGELA DE ALENCAR MARQUES; JOSÉ VICENTE TAGLIARINI; EMANUEL CELICE CASTILHO; PAULA SOARES; GLÁUCIA MARIA FERREIRA DA SILVA MAZETO. Follicular Lesions with Papillary Nuclear Characteristics: Differences in Chromatin Detected by Computerized Image Analysis. ARCHIVES OF ENDOCRINOLOGY METABOLISM, n. ahead, p. -, 2020.
BÁRBARA PARENTE COELHO; FLÁVIA DE OLIVEIRA VALENTIM; HÉLIO AMANTE MIOT; DANILO TAKESHI ABE JAUNE; CAROLINE YUKI HAYASHI; CRISTIANO CLAUDINO DE OLIVEIRA; MARIÂNGELA DE ALENCAR MARQUES; JOSÉ VICENTE TAGLIARINI; EMANUEL CELICE CASTILHO; PAULA SOARES; GLÁUCIA MARIA FERREIRA DA SILVA MAZETO. Follicular Lesions with Papillary Nuclear Characteristics: Differences in Chromatin Detected by Computerized Image Analysis. ARCHIVES OF ENDOCRINOLOGY METABOLISM, v. 64, n. 5, p. 630-635, Out. 2020.
VALENTIM, FLAVIA O.; COELHO, BARBARA P.; MIOT, HELIO A.; HAYASHI, CAROLINE Y.; JAUNE, DANILO T. A.; OLIVEIRA, CRISTIANO C.; MARQUES, MARIANGELA E. A.; TAGLIARINI, JOSE VICENTE; CASTILHO, EMANUEL C.; SOARES, PAULA; MAZETO, GLAUCIA M. F. S. Follicular thyroid lesions: is there a discriminatory potential in the computerized nuclear analysis?. ENDOCRINE CONNECTIONS, v. 7, n. 8, p. 907-913, AUG 2018. Web of Science Citations: 1.

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