Detection of an untyped strain of bovine respiratory syncytial virus in a dairy herd

Autor(es)

Affonso, Ingrid Bortolin ^[1] ; de Souza, Andressa ^[2] ; Martini, Matheus Cavalheiro ^[3] ; Aparecida Bianchi dos Santos, Marcia Merces ^[4] ; Spilki, Fernando Rosado ^[5] ; Arns, Clarice Weis ^[3] ; Samara, Samir Issa ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dept Med Vet Prevent & Reprod Anim, Programa Posgrad Med Vet, UNESP, Fac Ciencias Agr & Vet, Jaboticabal, SP - Brazil

^[2] UNESP, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, UNICAMP, Campinas, SP - Brazil

^[4] Univ Fed Juiz de Fora, Dept Parasitol Microbiol & Imunol, UFJF, Juiz De Fora, MG - Brazil

^[5] Univ FEEVALE, FEEVALE, Novo Hamburgo, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SEMINA-CIENCIAS AGRARIAS; v. 35, n. 5, p. 2539-2549, 2014.
• Citações Web of Science: 3

Resumo

Bovine respiratory syncytial virus (BRSV) causes important lower respiratory tract illness in calves. According to F and G proteins genetic sequences, three BRSV subgroups have been reported and characterized in several countries, showing differences in its distribution. In Brazil, the virus is widely disseminated throughout the herds and the few characterized isolates revealed the solely occurrence of the subgroup B. This study describes the detection and characterization of an untyped BRSV strain from a twenty-days-old calf from a herd without clinical respiratory disease. Nasal swabs were analyzed by RT-nested PCR for the F and G proteins genes. One sample has amplified the F protein gene. Sequencing and subsequent phylogenetic reconstruction were accomplished, revealing that the strain could not be grouped with any other BRSV subgroups reported. This result may suggest that the BRSV is in constantly evolution, even in Brazil, where the vaccination is not a common practice. More detailed studies about BRSV characterization are necessary to know the virus subgroups distribution among the Brazilian herds to recommend appropriated immunoprophylaxis. (AU)

• Processo FAPESP: 10/06950-2 - Caracterização epidemiológica e riscos associados ao vírus respiratório sincicial bovino (brsv) em rebanhos bovinos do estado de são paulo
• Beneficiário: Ingrid Bortolin Affonso Lux Hoppe
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/15912-7 - Caracterização epidemiológica e riscos associados ao vírus respiratório sincicial bovino (brsv) em rebanhos bovinos do estado de são paulo
• Beneficiário: Samir Issa Samara
• Modalidade de apoio: Auxílio à Pesquisa - Regular