Metalloproteomic Profile Determination of Muscle Samples from Nile Tilapia (Oreochromis niloticus) Using AAS and ESI-MS/MS after 2D-PAGE Separation

Autor(es)

Cavecci, Bruna ^[1] ; de Lima, Paula M. ^[1] ; de Queiroz, Joao V. ^[1] ; Braga, Camila P. ^[2] ; Padilha, Cilene C. F. ^[2] ; Leite, Aline L. ^[3] ; Buzalaf, Marilia A. R. ^[3] ; Pezzato, Luiz E. ^[1] ; Padilha, Pedro M. ^[2]

Afiliação do(s) autor(es)

^[1] UNESP, Coll Vet & Anim Sci, BR-18618970 Botucatu, SP - Brazil

^[2] UNESP, Inst Biosci, Dept Chem & Biochem, BR-18618970 Botucatu, SP - Brazil

^[3] UNESP, Dept Biol Sci, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 26, n. 2, p. 239-246, FEB 2015.
• Citações Web of Science: 2

Resumo

This study evaluated the metalloproteomic profile of muscle tissue samples from Nile tilapia (Oreochromis niloticus). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) methods was used for protein fractionation and identification based on image analysis. Determination of Ca, Cu, Fe, Mn and Zn was performed in the protein spots by flame and/or graphite furnace atomic absorption spectrometry (FAAS, GFAAS), the protein spots were characterized by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). FAAS and GFAAS determinations have indicated the presence of calcium in seven protein spots, iron in only one spot, manganese in six spots, and zinc in two spots. Of the seventeen protein spots where the presence of metal ions was detected, ten were characterized by ESI-MS/MS. (AU)

• Processo FAPESP: 10/51332-5 - Desenvolvimento de métodos analíticos para estudo metalômico do mercúrio em peixes coletados na área de influência do AHE Jirau Bacia do Rio Madeira
• Beneficiário: Pedro de Magalhães Padilha
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/14902-0 - Caracterização de metaloproteínas por espectrometria de massas (EASI-MS) em amostras de Tilápia do Nilo (Oreochromis niloticus)
• Beneficiário: Bruna Cavecci Mendonça
• Modalidade de apoio: Bolsas no Brasil - Mestrado