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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Metalloproteomic Profile Determination of Muscle Samples from Nile Tilapia (Oreochromis niloticus) Using AAS and ESI-MS/MS after 2D-PAGE Separation

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Author(s):
Cavecci, Bruna [1] ; de Lima, Paula M. [1] ; de Queiroz, Joao V. [1] ; Braga, Camila P. [2] ; Padilha, Cilene C. F. [2] ; Leite, Aline L. [3] ; Buzalaf, Marilia A. R. [3] ; Pezzato, Luiz E. [1] ; Padilha, Pedro M. [2]
Total Authors: 9
Affiliation:
[1] UNESP, Coll Vet & Anim Sci, BR-18618970 Botucatu, SP - Brazil
[2] UNESP, Inst Biosci, Dept Chem & Biochem, BR-18618970 Botucatu, SP - Brazil
[3] UNESP, Dept Biol Sci, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 26, n. 2, p. 239-246, FEB 2015.
Web of Science Citations: 2
Abstract

This study evaluated the metalloproteomic profile of muscle tissue samples from Nile tilapia (Oreochromis niloticus). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) methods was used for protein fractionation and identification based on image analysis. Determination of Ca, Cu, Fe, Mn and Zn was performed in the protein spots by flame and/or graphite furnace atomic absorption spectrometry (FAAS, GFAAS), the protein spots were characterized by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). FAAS and GFAAS determinations have indicated the presence of calcium in seven protein spots, iron in only one spot, manganese in six spots, and zinc in two spots. Of the seventeen protein spots where the presence of metal ions was detected, ten were characterized by ESI-MS/MS. (AU)

FAPESP's process: 10/51332-5 - Development of analytical methods for mercury metallomics studies in fish collected in the AHE JIRAU - Madeira River Basin area of influence
Grantee:Pedro de Magalhães Padilha
Support Opportunities: Regular Research Grants
FAPESP's process: 11/14902-0 - Metalloproteins characterization by electrospray ionization mass spectrometry (ESI-MS) in samples of Nile Tilapia (Oreochromis niloticus)
Grantee:Bruna Cavecci Mendonça
Support Opportunities: Scholarships in Brazil - Master