Regulation of Leishmania (L.) amazonensis Protein Expression by Host T Cell Dependent Responses: Differential Expression of Oligopeptidase B, Tryparedoxin Peroxidase and HSP70 Isoforms in Amastigotes Isolated from BALB/c and BALB/c Nude Mice

Autor(es)

Teixeira, Priscila Camillo ^[1] ; Velasquez, Leonardo Garcia ^[2] ; Lepique, Ana Paula ^[3] ; de Rezende, Eloiza ^[2] ; Camargo Bonatto, Jose Matheus ^[4] ; Barcinski, Marcello Andre ^[5] ; Cunha-Neto, Edecio ^[1] ; Stolf, Beatriz Simonsen ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Heart Inst InCor, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil

^[5] Oswaldo Cruz Inst FioCruz, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS Neglected Tropical Diseases; v. 9, n. 2 FEB 2015.
• Citações Web of Science: 11
• Assunto(s): Etiologia Leishmaniose cutânea Infecções por HIV

Resumo

Leishmaniasis is an important disease that affects 12 million people in 88 countries, with 2 million new cases every year. Leishmania amazonensis is an important agent in Brazil, leading to clinical forms varying from localized (LCL) to diffuse cutaneous leishmaniasis (DCL). One interesting issue rarely analyzed is how host immune response affects Leishmania phenotype and virulence. Aiming to study the effect of host immune system on Leishmania proteins we compared proteomes of amastigotes isolated from BALB/c and BALB/c nude mice. The athymic nude mice may resemble patients with diffuse cutaneous leishmaniasis, considered T-cell hyposensitive or anergic to Leishmania's antigens. This work is the first to compare modifications in amastigotes' proteomes driven by host immune response. Among the 44 differentially expressed spots, there were proteins related to oxidative/nitrosative stress and proteases. Some correspond to known Leishmania virulence factors such as OPB and tryparedoxin peroxidase. Specific isoforms of these two proteins were increased in parasites from nude mice, suggesting that T cells probably restrain their posttranslational modifications in BALB/c mice. On the other hand, an isoform of HSP70 was increased in amastigotes from BALB/c mice. We believe our study may allow identification of potential virulence factors and ways of regulating their expression. (AU)

• Processo FAPESP: 08/56923-1 - Expressao proteica em amastigotas de leishmania amazonensis: modulacao pela resposta imune de camundongos experimentalmente infectados.
• Beneficiário: Beatriz Simonsen Stolf
• Modalidade de apoio: Auxílio à Pesquisa - Regular