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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Molecular diagnosis of cryptococcal meningitis in cerebrospinal fluid: comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex

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Autor(es):
Martins, Marilena dos Anjos [1, 2] ; Santos Brighente, Kate Bastos [1] ; de Matos, Terezinha Aparecida [3] ; Vidal, Jose Ernesto [4] ; Carnietto de Hipolito, Daise Damaris [2] ; Pereira-Chioccola, Vera Lucia [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Adolfo Lutz Inst, Lab Biol Mol Parasitas & Fungos, BR-01246902 Sao Paulo - Brazil
[2] Adolfo Lutz Inst, Nucleo Micol, BR-01246902 Sao Paulo - Brazil
[3] Inst Infectol Emilio Ribas, Lab Liquido Cefalorraquiano, Sao Paulo - Brazil
[4] Inst Infectol Emilio Ribas, Dept Neurol, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Infectious Diseases; v. 19, n. 1, p. 62-67, JAN-FEB 2015.
Citações Web of Science: 4
Resumo

Aim: This study evaluated the use of polymerase chain reaction for cryptococcal meningitis diagnosis in clinical samples. Materials and methods: The sensitivity and specificity of the methodology were evaluated using eight Cryptococcus neoformans/C. gattii species complex reference strains and 165 cerebrospinal fluid samples from patients with neurological diseases divided into two groups: 96 patients with cryptococcal meningitis and AIDS; and 69 patients with other neurological opportunistic diseases (CRL/AIDS). Two primer sets were tested (CN4-CN5 and the multiplex CNa70S-CNa70A/CNb49S-CNb-49A that amplify a specific product for C. neoformans and another for C. gattii). Results: CN4-CN5 primer set was positive in all Cryptococcus standard strains and in 94.8% in DNA samples from cryptococcal meningitis and AIDS group. With the multiplex, no 448-bp product of C. gattii was observed in the clinical samples of either group. The 695 bp products of C. neoformans were observed only in 64.6% of the czyptococcal meningitis and AIDS group. This primer set was negative for two standard strains. The specificity based on the negative samples from the CTL/AIDS group was 98.5% in both primer sets. Conclusions: These data suggest that the CN4/CN5 primer set was highly sensitive for the identification of C. neoformans/C. gotta species complex in cerebrospinal fluid samples from patients with clinical suspicion of cryptococcal meningitis. (C) 2014 Elsevier Editora Ltda. Este e um artigo Open Access sob a licenca de CC BY-NC-ND (AU)

Processo FAPESP: 11/13939-8 - Estudo de um grupo de infecções fúngico-parasitárias visando a melhoria das técnicas de caracterização e diagnóstico imuno-molecular
Beneficiário:Vera Lúcia Pereira Chioccola
Linha de fomento: Auxílio à Pesquisa - Regular