Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation

Autor(es)

Lima, Gisele Negro ^[1] ; Maganhin, Carla Cristina ^[1] ; Simoes, Ricardo Santos ^[2] ; Pinheiro Baracat, Maria Candida ^[2] ; da Silva Sasso, Gisela Rodrigues ^[3] ; Portugal Fuchs, Luiz Fernando ^[2] ; Simoes, Manuel de Jesus ^[3] ; Baracat, Edmund Chada ^[2] ; Soares Junior, Jose Maria ^[2]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo EPM UNIFESP, Paulista Sch Med, Dept Gynecol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo FMUSP USP, Fac Med, Dept Obstet & Gynecol, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, EPM UNIFESP, Paulista Sch Med, Dept Morphol & Genet, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinics; v. 70, n. 2, p. 144-151, 2015.
• Citações Web of Science: 8

Resumo

OBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 mu g/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip (R) Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip (R) Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis. (AU)

• Processo FAPESP: 11/51581-8 - Análise de melatonina em mulheres com infertilidade submetidas a um programa de fertilização in vitro: estudo funcional nas células da granulosa
• Beneficiário: Carla Cristina Maganhin
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado