Hemicellulosic ethanol production by immobilized cells of Scheffersomyces stipitis: Effect of cell concentration and stirring

Autor(es)

Milessi, Thais S. S. ^[1] ; Antunes, Felipe A. F. ^[1] ; Chandel, Anuj K. ^[1] ; da Silva, Silvio S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Engn Sch Lorena, Dept Biotechnol, Lorena - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOENGINEERED; v. 6, n. 1, p. 26-32, JAN-FEB 2015.
• Citações Web of Science: 7

Resumo

Bioconversion of hemicellulosic hydrolysate into ethanol plays a pivotal role in the overall success of biorefineries. For the efficient fermentative conversion of hemicellulosic hydrolysates into ethanol, the use of immobilized cells system could provide the enhanced ethanol productivities with significant time savings. Here, we investigated the effect of 2 important factors (e.g., cell concentration and stirring) on ethanol production from sugarcane bagasse hydrolysate using the yeast Scheffersomyces stipitis immobilized in calcium alginate matrix. A 2(2) full factorial design of experiment was performed considering the process variables- immobilized cell concentration (3.0, 6.5 and 10.0g/L) and stirring (100, 200 and 300rpm). Statistical analysis showed that stirring has the major influence on ethanol production. Maximum ethanol production (8.90g/l) with ethanol yield (Yp/s) of 0.33g/g and ethanol productivity (Qp) of 0.185g/l/h was obtained under the optimized process conditions (10.0g/L of cells and 100rpm). (AU)

• Processo FAPESP: 08/57926-4 - Etanol: pesquisa e desenvolvimento visando ao aproveitamento integrado do bagaço de cana-de-açúcar para a produção biotecnológica do etanol lignocelulósico
• Beneficiário: Silvio Silvério da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 10/13828-9 - Processo fermentativo de obtenção de etanol de segunda geração a partir de hidrolisado hemicelulósico de bagaço de cana-de-açúcar em biorreator STR tipo cesta operado com células imobilizadas
• Beneficiário: Thais Suzane Milessi Esteves
• Modalidade de apoio: Bolsas no Brasil - Mestrado