The Prelimbic Cortex Muscarinic M-3 Receptor-Nitric Oxide-Guanylyl Cyclase Pathway Modulates Cardiovascular Responses in Rats

Autor(es)

Fassini, Aline ^[1] ; Antero, Leandro S. ^[1] ; Correa, Fernando M. A. ^[1] ; Joca, Samia R. ^[2] ; Resstel, Leonardo B. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pharmacol, Sch Med Ribeirao Preto, BR-14049900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Phys & Chem, Pharmacol Lab, Sch Pharmaceut Sci Ribeirao Preto, BR-14049900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Neuroscience Research; v. 93, n. 5, p. 830-838, MAY 2015.
• Citações Web of Science: 5

Resumo

The prelimbic cortex (PL), a limbic structure, sends projections to areas involved in the control of cardiovascular responses. Stimulation of the PL with acetylcholine (ACh) evokes depressor and tachycardiac responses mediated by local PL muscarinic receptors. Early studies demonstrated that stimulation of muscarinic receptors induced nitric oxide (NO) synthesis and cyclic guanosine cyclic monophosphate (cGMP) formation. Hence, this study investigates which PL muscarinic receptor subtype is involved in the cardiovascular response induced by ACh and tests the hypothesis that cardiovascular responses caused by muscarinic receptor stimulation in the PL are mediated by local NO and cGMP formation. PL pretreatment with J104129 (an M-3 receptor antagonist) blocked the depressor and tachycardiac response evoked by injection of ACh into the PL. Pretreatment with either pirenzepine (an M-1 receptor antagonist) or AF-DX 116 (an M-2 and M-4 receptor antagonist) did not affect cardiovascular responses evoked by ACh. Moreover, similarly to the antagonism of PL M-3 receptors, pretreatment with Nxpropyl- L-arginine (an inhibitor of neuronal NO synthase), carboxy-PTIO(S)-3-carboxy-4-hydroxyphenylglicine (an NO scavenger), or H-1-{[}1,2,4] oxadiazolol-{[}4,3-a] quinoxalin-1- one (a guanylate cyclase inhibitor) blocked both the depressor and the tachycardiac response evoked by ACh. The current results demonstrate that cardiovascular responses evoked by microinjection of ACh into the PL are mediated by local activation of the M-3 receptor-NO- guanylate cyclase pathway. (C) 2015 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 12/17626-7 - Mecanismos celulares e moleculares envolvidos no papel de neurotransmissores atípicos em transtornos neuropsiquiátricos
• Beneficiário: Francisco Silveira Guimaraes
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/09300-4 - O sistema noradrenérgico presente no núcleo leito da estria terminal modula a resposta emocional condicionada contextual: uma possível interação com o CRF e as neurotransmissões glutamatérgica e nitrérgica
• Beneficiário: Leonardo Resstel Barbosa Moraes
• Linha de fomento: Auxílio à Pesquisa - Regular