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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

CXCR7 and CXCR4 Expressions in Infiltrative Astrocytomas and Their Interactions with HIF1 alpha Expression and IDH1 Mutation

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Autor(es):
Bianco, Andre Macedo [1] ; Uno, Miyuki [1] ; Oba-Shinjo, Sueli Mieko [1] ; Clara, Carlos Afonso [2] ; de Almeida Galatro, Thais Fernanda [1] ; Rosemberg, Sergio [3] ; Teixeira, Manoel Jacobsen [3, 1] ; Nagahashi Marie, Suely Kazue [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Dept Neurol, Lab Mol & Cellular Biol, BR-01246903 Sao Paulo, SP - Brazil
[2] State Sao Paulo, Canc Hosp Barretos, Pio Fundat 12, BR-14784400 Barretos, SP - Brazil
[3] Univ Sao Paulo, Sch Med, Dept Pathol, BR-01246903 Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Pathology & Oncology Research; v. 21, n. 2, p. 229-240, APR 2015.
Citações Web of Science: 5
Resumo

The CXCR7, a new receptor for CXCL12 with higher affinity than CXCR4 has raised key issues on glioma cell migration. The aim of this study is to investigate the CXCR7 mRNA expression in diffuse astrocytomas tissues and to evaluate its interactions with CXCR4 and HIF1 alpha expression and IDH1 mutation. CXCR7, CXCR4 and HIF1 alpha mRNA expression were evaluated in 129 frozen samples of astrocytomas. IDH1 mutation status was analyzed with gene expressions, matched with clinicopathological parameters and overall survival time. Protein expression was analyzed by immunohistochemistry in different grades of astrocytoma and in glioma cell line (U87MG) by confocal microscopy. There was significant difference in the expression levels of the genes studied between astrocytomas and non-neoplasic (NN) controls (p < 0.001). AGII showed no significant correlation between CXCR7/HIF1 alpha (p = 0.548); there was significant correlation between CXCR7/CXCR4 (p = 0.042) and CXCR7/IDH1 (p = 0.008). GBM showed significant correlations between CXCR7/CXCR4 (p = 0.002), CXCR7/IDH1 (p < 0.001) and CXCR7/HIF1 alpha (p = 0.008). HIF1 alpha overexpression was associated with higher expressions of CXCR7 (p = 0.01) and CXCR4 (p < 0.0001), while IDH1 mutation was associated with lower CXCR7 (p = 0.009) and CXCR4 (p = 0.0005) mRNA expressions. Protein expression increased with malignancy and in U87MG cell line was mainly localized in the cellular membrane. CXCR7 was overexpressed in astrocytoma and correlates with CXCR4 and IDH1 in AGII and CXCR4, IDH1 and HIF1 alpha in GBM. Overexpression HIF1 alpha was related with higher expressions of CXCR7 and CXCR4, otherwise IDH1 mutation related with lower expression of both genes. No association between CXCR7 and CXCR4 expression and survival data was related. (AU)

Processo FAPESP: 01/12898-4 - Genoma clínico
Beneficiário:Suely Kazue Nagahashi Marie
Linha de fomento: Auxílio à Pesquisa - Programa GENOMA
Processo FAPESP: 04/12133-6 - Procura de marcadores moleculares relacionados ao diagnóstico e prognóstico de tumores do sistema nervoso central
Beneficiário:Suely Kazue Nagahashi Marie
Linha de fomento: Auxílio à Pesquisa - Temático