Evaluation of Testicular Tissue of Adult Rats Treated With Cisplatin Incorporated Into the Liposome

Autor(es)

Alves Favareto, Ana Paula ^[1] ; Nogueira, Lazaro Cicero ^[2] ; Pontes, Davi Abeid ^[2] ; Paccola Mesquita, Suzana De Fatima ^[3] ; Cherici Camargo, Isabel Cristina ^[2]

Afiliação do(s) autor(es)

^[1] Univ Western Sao Paulo UNOESTE, Grad Program Environm & Reg Dev, Sao Paulo - Brazil

^[2] Univ Estadual Paulista UNESP, Fac Sci & Letters, Dept Biol Sci, BR-19806900 Sao Paulo - Brazil

^[3] State Univ Londrina UEL, Dept Gen Biol, Londrina, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 78, n. 4, p. 323-329, APR 2015.
• Citações Web of Science: 2

Resumo

Cisplatin (CPL) is one of the most widely used and effective chemotherapeutic agents for the treatment of several human malignancies. However, it causes serious side effects, especially on reproduction. In order to reduce the undesirable effects caused by many drugs, liposomes have been used as a good system for drug delivery. The aim of this study was to investigate, for the first time, the effects of CPL incorporated into the dipalmitoyl phosphatidylcholine liposome (DPPC) on the testicular tissue of adult Wistar rats. The animals (n=20) were distributed into four experimental groups: (a) control (distillated water); (b) liposome (DPPC, 1 mL), (c) cisplatin incorporated into liposome (CPL/DPPC), and (d) CPL (8 mg/kg body weight). The animals received a single intraperitoneal injection and were killed 10 days after each treatment for histopathological analysis of testes. The results showed that the testicular histomorphometric parameters in rats of DPPC and CPL/DPPC groups were similar to those of the control group. Meanwhile, rats of the CPL-treated group showed a variety of morphological alterations, including atrophy of seminiferous tubules and presence of multinucleated cells in the germinal epithelium. The incorporation of CPL into the liposome had no influence on the testicular weight or any other stereological parameters, but it was beneficial in maintaining the body weight of the animals. In conclusion, the liposome suppressed the cytotoxic effects caused by cisplatin in the testes of rats, suggesting a possible use in chemotherapy against cancer to reduce the side effects seen on reproduction. Microsc. Res. Tech. 78:323-329, 2015. (c) 2015 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 04/01766-8 - Efeito da interação do agente oncolítico cisplatina ao lipossoma dipalmitoil fosfatidil colina sobre aspectos morfológicos e morfométricos de testículos de ratos adultos
• Beneficiário: Ana Paula Alves Favareto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica