Genomic and chemical insights into biosurfactant production by the mangrove-derived strain Bacillus safensis CCMA-560

Autor(es)

Domingos, Daniela Ferreira ^[1] ; de Faria, Andreia Fonseca ^[2] ; Galaverna, Renan de Souza ^[3] ; Eberlin, Marcos Nogueira ^[3] ; Greenfield, Paul ^[4] ; Zucchi, Tiago Domingues ^[5] ; Melo, Itamar Soares ^[5] ; Tran-Dinh, Nai ^[6] ; Midgley, David ^[6] ; de Oliveira, Valeria Maia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Microbial Resources Div, Res Ctr Chem Biol & Agr CPQBA, BR-13148218 Campinas, SP - Brazil

^[2] Yale Univ, Dept Chem & Environm Engn, New Haven, CT 06520 - USA

^[3] Univ Campinas UNICAMP, ThoMSon Mass Spectrometry Lab, Inst Chem, BR-13148218 Campinas, SP - Brazil

^[4] CSIRO Math Informat & Stat, N Ryde, NSW 2113 - Australia

^[5] EMBRAPA Environm, Lab Environm Microbiol, Sao Paulo - Brazil

^[6] Nai Tran-Dinh, CSIRO Anim Food & Hlth Sci, N Ryde, NSW 2113 - Australia

• Tipo de documento: Artigo Científico
• Fonte: Applied Microbiology and Biotechnology; v. 99, n. 7, p. 3155-3167, APR 2015.
• Citações Web of Science: 11

Resumo

Many Bacillus species can produce biosurfactant, although most of the studies on lipopeptide production by this genus have been focused on Bacillus subtilis. Surfactants are broadly used in pharmaceutical, food and petroleum industry, and biological surfactant shows some advantages over the chemical surfactants, such as less toxicity, production from renewable, cheaper feedstocks and development of novel recombinant hyperproducer strains. This study is aimed to unveil the biosurfactant metabolic pathway and chemical composition in Bacillus safensis strain CCMA-560. The whole genome of the CCMA-560 strain was previously sequenced, and with the aid of bioinformatics tools, its biosurfactant metabolic pathway was compared to other pathways of closely related species. Fourier transform infrared (FTIR) and high-resolution TOF mass spectrometry (MS) were used to characterize the biosurfactant molecule. B. safensis CCMA-560 metabolic pathway is similar to other Bacillus species; however, some differences in amino acid incorporation were observed, and chemical analyses corroborated the genetic results. The strain CCMA-560 harbours two genes flanked by srfAC and srfAD not present in other Bacillus spp., which can be involved in the production of the analogue gramicidin. FTIR and MS showed that B. safensis CCMA-560 produces a mixture of at least four lipopeptides with seven amino acids incorporated and a fatty acid chain with 14 carbons, which makes this molecule similar to the biosurfactant of Bacillus pumilus, namely, pumilacidin. This is the first report on the biosurfactant production by B. safensis, encompassing the investigation of the metabolic pathway and chemical characterization of the biosurfactant molecule. (AU)

• Processo FAPESP: 11/50243-1 - Diversidade e taxonomia polifásica de actinobactérias isoladas de áreas de reflorestamento
• Beneficiário: Tiago Domingues Zucchi
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Jovens Pesquisadores
• Processo FAPESP: 11/50809-5 - Prospecção de metagenoma microbiano de sedimentos de manguezais na busca por novos compostos bioativos
• Beneficiário: Valeria Maia Merzel
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 10/15519-3 - Prospecção de biossurfactantes a partir de microbiota de manguezais
• Beneficiário: Daniela Ferreira Domingos
• Modalidade de apoio: Bolsas no Brasil - Doutorado