Genetic variants in glucocorticoid and mineralocorticoid receptors are associated with concentrations of plasma cortisol, muscle glycogen content, and meat quality traits in male Nellore cattle

Autor(es)

Poleti, M. D. ^[1] ; DeRijk, R. H. ^{[2, 3]} ; Rosa, A. F. ^[1] ; Moncau, C. T. ^[1] ; Oliveira, P. S. ^[1] ; Coutinho, L. L. ^[4] ; Eler, J. P. ^[1] ; Balieiro, J. C. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Anim Sci & Food Engn, Dept Basic Sci, BR-13635900 Sao Paulo - Brazil

^[2] Leiden Univ, Med Ctr, Leiden Amsterdam Ctr Drug Res, Div Med Pharmacol, Leiden - Netherlands

^[3] Leiden Univ, Med Ctr, Dept Psychiat, Leiden - Netherlands

^[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Anim Sci, BR-13418900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DOMESTIC ANIMAL ENDOCRINOLOGY; v. 51, p. 105-113, APR 2015.
• Citações Web of Science: 7

Resumo

The glucocorticoid receptor (GR) and, mineralocorticoid receptor (MR) are key components in the regulation of the hypothalamic-pituitary-adrenal neuroendocrine axis and coordinate the physiological response to stress agents to reestablish homeostasis. Genetic variations of GR (NR3C1) and MR (NR3C2) genes could explain the alterations in animals to adapt to challenges, and therefore, their influence on production traits. The present study aimed to identify single-nucleotide polymorphisms (SNPs) in the bovine NR3C1 and NR3C2 genes and explore their associations to relevant traits of beef cattle production. Genotypes and phenotypes were collected from 241 male Nellore cattle (119 noncastrated and 122 castrated surgically) with an average of 24 +/- 1.2 mo of age and live weight of 508 +/- 39 kg. The traits evaluated were concentrations of plasma adrenocorticotropic hormone (ACTH) and cortisol, muscle glycogen and lactate content, and pH, color, cooking loss, and shear force of longissimus thoracis measured on the 1st, 7th, and 14th days postmortem. Five SNPs were identified, 2 in the NR3C1 gene and 3 in the NR3C2 gene. There was an associative relationship between the SNP NR3C1\_1 g.3293A>G and postmortem plasma concentration of cortisol (P = 0.0008). The SNPs NR3C2\_1 g.115T>C and NR3C2\_2 g.570T>C were associated with muscle glycogen content (P = 0.0306 and P = 0.0158), postmortem plasma concentration of ACTH (P = 0.0118 and P = 0.0.095), and cooking loss of the steak aged 1 d (P = 0.0398 and P = 0.0423). Haplotype analysis showed associations of GR haplotypes with postmortem plasma concentrations of cortisol and MR haplotypes with meat color, cooking losses, muscle glycogen content, and plasma concentrations of ACTH. The associations observed in the present study show that SNPs in GR and MR genes are related with changes of hypothalamic-pituitary-adrenal axis activity and metabolic profile in cattle, leading to individual variation in meat quality traits. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 10/07057-0 - Variação nos genes dos receptores mineralocorticóide e glucocorticóide e suas implicações proteômicas na qualidade da carne de bovinos Nelore
• Beneficiário: Mirele Daiana Poleti
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/07078-7 - Variação nos genes dos receptores mineralocorticóide e glucocorticóide e implicações proteômicas na qualidade da carne de bovinos Nelore
• Beneficiário: Júlio Cesar de Carvalho Balieiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular