Effect of inulin on growth and bacteriocin production by Lactobacillus plantarum in stationary and shaken cultures

Autor(es)

Sabo, Sabrina da Silva ^[1] ; Converti, Attilio ^[2] ; Todorov, Svetoslav Dimitrov ^[3] ; Dominguez, Jose Manuel ^[4] ; de Souza Oliveira, Ricardo Pinheiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, Fac Pharmaceut Sci, Sao Paulo - Brazil

^[2] Univ Genoa, Pole Chem Engn, Dept Civil Chem & Environm Engn, Genoa - Italy

^[3] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo - Brazil

^[4] Univ Vigo, Dept Chem Engn, Fac Sci, Orense 32004 - Spain

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY; v. 50, n. 4, p. 864-870, APR 2015.
• Citações Web of Science: 10

Resumo

The prebiotic effect of inulin added to MRS medium on growth and bacteriocin production by L.plantarum ST16 Pa was investigated in stationary cultures in anaerobic jars with medium containing 0.025% sodium thioglycolate or in flasks shaken at 100rpm. In the presence of 1% inulin in anaerobic stationary cultures, this strain produced lactic acid at a level that was 36.5% higher than in the absence of the polysaccharide. In shaken cultures without inulin, cell count was 54% higher than in the stationary ones. Under stationary conditions in anaerobic jars, the addition of inulin increased the maximum specific growth rate from 0.37 to 0.49h(-1) and reduced the generation time from 1.85h to 1.40h. Consequently, the exponential phase was shortened from 12 to 9h when the cells were grown in stationary cultures with the oxygen scavenger. Despite this effect of inulin on growth rate, stationary cultures without inulin displayed higher antimicrobial activity against Listeria monocytogenes L104 (3200AU/mL) than cultures with inulin (1600AU/mL); therefore, inulin behaved as a compound able to accelerate growth rather than to stimulate bacteriocin production. The results presented in this study are very promising, as L.monocytogenes is a well-known foodborne pathogenic microorganism. Moreover, L.plantarum ST16 Pa has proven to be a potential producer of a natural food preservative at an industrial level. (AU)

• Processo FAPESP: 12/23340-9 - A influência da inulina no crescimento celular de Lactobacillus plantarum ST16Pa e concomitante produção de bacteriocina a partir do caldo MRS e soro de leite
• Beneficiário: Sabrina da Silva Sabo
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/19997-5 - Processo de internacionalização em pesquisa biotecnológica entre as Universidades de São Paulo e Vigo
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
• Processo FAPESP: 13/12713-1 - Produção biotecnológica e purificação de plantaricina por Lactobacillus plantarum St16Pa utilizando meio de cultivo natural
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular