High organic loading rate on thermophilic hydrogen production and metagenomic study at an anaerobic packed-bed reactor treating a residual liquid stream of a Brazilian biorefinery

Autor(es)

Nunes Ferraz Junior, Antonio Djalma ^{[1, 2]} ; Etchebehere, Claudia ^[3] ; Zaiat, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Sch Engn EESC, Ctr Res Dev & Innovat Environm Engn, Biol Proc Lab, BR-13563120 Sao Carlos, SP - Brazil

^[2] CNPEM, CTBE Brazilian Bioethanol Sci & Technol Lab, BR-13083970 Campinas, SP - Brazil

^[3] Inst Invest Biol Clemente Estable, Lab Ecol Microbiana, Montevideo - Uruguay

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 186, p. 81-88, JUN 2015.
• Citações Web of Science: 27

Resumo

This study evaluated the influence of a high organic loading rate (OLR) on thermophilic hydrogen production at an up-flow anaerobic packed-bed reactor (APBR) treating a residual liquid stream of a Brazilian biorefinery. The APBR, filled with low-density polyethylene, was operated at an OLR of 84.2 kg-COD m(-3) d(-1). This value was determined in a previous study. The maximum values of hydrogen production and yield were 5,252.6 mL-H-2 d(-1) and 3.7 mol-H-2 mol(total carbohydrates)(-1), respectively. However, whereas the OLR remained constant, the specific organic load rate (sOLR) decreased throughout operation from 1.38 to 0.72 g-Total carbohydrates g-VS-1 h(-1), this decrease negatively affected hydrogen production. A sOLR of 0.98 g-Total carbohydrates g-VS-1 h(-1) was optimal for hydrogen production. The microbial community was studied using 454-pyrosequencing analysis. Organisms belonging to the genera Caloramator, Clostridium, Megasphaera, Oxobacter, Thermoanaerobacterium, and Thermohydrogenium were detected in samples taken from the reactor at operation days 30 and 60, suggesting that these organisms contribute to hydrogen production. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/15984-0 - Produção de bioenergia no tratamento de águas residuárias e adequação ambiental dos efluentes e resíduos gerados
• Beneficiário: Marcelo Zaiat
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/15665-8 - Estrutura e dinâmica funcional da comunidade microbiana de digestores anaeróbios, aplicados à recuperação de energia e adequação ambiental de resíduos gerados no processo produtivo do bioetanol de 1ª e 2ª geração
• Beneficiário: Antônio Djalma Nunes Ferraz Júnior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado