Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Acetone as a greener alternative to acetonitrile in liquid chromatographic fingerprinting

Texto completo
Autor(es):
Funari, Cristiano Soleo [1, 2, 3] ; Carneiro, Renato Lajarim [4] ; Khandagale, Manish M. [2] ; Cavalheiro, Alberto Jose [1] ; Hilder, Emily F. [2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Inst Chem, Sao Paulo - Brazil
[2] Univ Tasmania, Sch Phys Sci, Australian Ctr Res Separat Sci ACROSS, Hobart, Tas - Australia
[3] Sao Paulo State Univ, Fac Agr Sci, Sao Paulo - Brazil
[4] Univ Fed Sao Carlos, Dept Chem, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF SEPARATION SCIENCE; v. 38, n. 9, p. 1458-1465, MAY 2015.
Citações Web of Science: 13
Resumo

A considerable amount of chemical waste from liquid chromatography analysis is generated worldwide. Acetonitrile is the most employed solvent in liquid chromatography analyses since it exhibits favorable physicochemical properties for separation and detection, but it is an unwelcome solvent from an environmental point of view. Acetone might be a much greener alternative to replace acetonitrile in reversed-phase liquid chromatography, since both share similar physicochemical properties, but its applicability with ultraviolet absorbance-based detectors is limited. In this work, a reference method using acetonitrile and high-performance liquid chromatography coupled to an ultraviolet photodiode array detector coupled to a corona charged aerosol detector system was developed to fingerprint a complex sample. The possibility of effectively substituting acetonitrile with acetone was investigated. Design of experiments was adopted to maximize the number of peaks acquired in both fingerprint developments. The methods with acetonitrile or acetone were successfully optimized and proved to be statistically similar when only the number of peaks or peak capacity was taken into consideration. However, the superiority of the latter was evidenced when parameters of separation and those related to greenness were heuristically combined. A green, comprehensive, time-and resource-saving approach is presented here, which is generic and applicable to other complex matrices. Furthermore, it is in line with environmental legislation and analytical trends. (AU)

Processo FAPESP: 12/15844-7 - Uma abordagem mais verde para o estudo do verde: cromatografia líquida sustentável aplicada ao estudo de perfis químicos de plantas medicinais brasileiras
Beneficiário:Cristiano Soleo de Funari
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
Beneficiário:Glaucius Oliva
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs