Characterization of Brown spider (Loxosceles intermedia) hemolymph: Cellular and biochemical analyses

Autor(es):Mostrar menos -

Bednaski, A. V. ^[1] ; Trevisan-Silva, D. ^[1] ; Matsubara, F. H. ^[1] ; Boia-Ferreira, M. ^[1] ; Oliverio, M. M. ^[1] ; Gremski, L. H. ^[2] ; Cavalheiro, R. P. ^[3] ; De Paula, D. M. B. ^[4] ; Paredes-Gamero, E. J. ^[3] ; Takahashi, H. K. ^[4] ; Toledo, M. S. ^[4] ; Nader, H. B. ^[3] ; Veiga, S. S. ^[1] ; Chaim, O. M. ^[1] ; Senff-Ribeiro, A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Parana, Dept Cell Biol, UFPR, BR-81531990 Curitiba, Parana - Brazil

^[2] Univ Fed Parana, Clin Hosp, Dept Clin Pathol, BR-81531990 Curitiba, Parana - Brazil

^[3] Univ Fed Sao Paulo, Discipline Mol Biol, Dept Biochem, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo, Lab Immunochem Glycoconjugates, Dept Biochem, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 98, p. 62-74, MAY 2015.
• Citações Web of Science: 3

Resumo

This is the first study on the hemolymph from a spider of the Loxosceles genus. These animals are responsible for a great number of envenomation cases worldwide. Several studies on Loxosceles venoms have been published, and the knowledge about the venom and its toxins is considerable, not only regarding the biological and biochemical characterization, but also regarding structural, genetic and phylogenetic approaches. However, the literature on Loxosceles hemolymph is nonexistent. The main goal of the present study was to characterize biochemically the hemolymph content, and especially, to identify its different hemocytes. Moreover, many papers have already shown molecules whose source is the hemolymph and their very interesting activities and biomedical applications, for example, antifungal and antibacterial activities. A 2D-SDS-PAGE of brown spider hemolymph showed approximately 111 spots for pH 3-10 and 150 spots for pH 4-7. A lectin-blotting assay showed that hemolymph carbohydrate residues were similar to those found in venom. Several types of TAG and DAG phospholipids were found in the hemolymph and characterized by HPTLC and mass spectrometry. Four different hemocytes were characterized in Loxosceles intermedia hemolymph: prohemocyte, plasmatocyte, granulocyte and adipohemocyte. This paper opens new possibilities on toxinology, studying an unknown biological material, and it characterizes a source of molecules with putative biotechnological applications. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 06/07005-4 - Microdomínios de membrana ricos em (glico) (esfingo) lipídeos e esteróis: organização, função e sinalização
• Beneficiário: Helio Kiyoshi Takahashi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/52426-3 - Espectrometria de massas e de ressonância magnética nuclear na caracterização estrutural de glicosaminoglicanos e polissacarídeos complexos de invertebrados e algas
• Beneficiário: Helena Bonciani Nader
• Modalidade de apoio: Auxílio à Pesquisa - Regular