Determination of pesticide residues in coconut tree trunks by modified QuEChERS method and ultra-high-performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry

Autor(es):Mostrar menos -

Ferreira, J. A. ^[1] ; Talamine, V. ^[2] ; Facco, J. F. ^[3] ; Rizzetti, T. M. ^[3] ; Ferreira, J. M. S. ^[2] ; Oliveira, F. A. ^[2] ; Prestes, O. D. ^[3] ; Zanella, R. ^[3] ; Martins, M. L. ^[3] ; Adaime, M. B. ^[3] ; Navickiene, S. ^[4] ; Bottoli, C. B. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP - Brazil

^[2] Embrapa Tabuleiros Costeiros, Dept Engn Agron, BR-49025040 Aracaju, SE - Brazil

^[3] Univ Fed Santa Maria, Dept Quim, BR-97105900 Santa Maria, RS - Brazil

^[4] Univ Fed Sergipe, Dept Quim, BR-49100000 Sao Cristovao, SE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL METHODS; v. 7, n. 10, p. 4237-4245, 2015.
• Citações Web of Science: 6

Resumo

A rapid and reliable method for the determination of 10 pesticide residues in coconut (Cocos nucifera L.) tree trunks after endotherapy treatments has been established. A modified QuEChERS (quick, easy, cheap, effective, rugged and safe) method, using an homogeneous sample slurry and acetate buffer, followed by ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Under the best extraction conditions, the average recoveries for all pesticides spiked at 40, 80 and 200 mu g kg(-1) ranged from 70 to 93%, with RSD <10%. Intermediate precision expressed as RSD, ranged between 3 and 6% for all compounds. Calibration curves showed a wide linear range between 10.0 and 1000.0 mu g kg(-1) for all compounds studied. Limit of quantification was established as 40.0 mu g kg(-1). The developed procedure was employed in the analysis of real coconut tree trunk samples obtained 45 h after pesticides application using endotherapy treatment. Concentrations of pesticides were between 44.7 +/- 5 and 938.3 +/- 20 mu g kg(-1). These results prove the translocation of pesticides in different heights, in the coconut tree trunk, from the application point. Imidacloprid presented the highest acropetal translocation and was found near the leaves at 61 +/- 6 mu g kg(-1). (AU)

• Processo FAPESP: 08/57805-2 - Instituto de Bioanalítica
• Beneficiário: Lauro Tatsuo Kubota
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/18318-4 - Avaliação da contaminação de coqueiros (Cocos nucifera L.) devido à translocação acropetal de agrotóxicos, por cromatografia líquida de alta eficiência
• Beneficiário: Jordana Alves Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado