Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)

Autor(es)

Davolos, Camila C. ^{[1, 2]} ; Hernandez-Martinez, Patricia ^[2] ; Crialesi-Legori, Paula C. B. ^[1] ; Desiderio, Janete A. ^[1] ; Ferre, Juan ^[2] ; Escriche, Baltasar ^[2] ; Lemos, Manoel Victor F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Appl Biol, BR-14884900 Jaboticabal, SP - Brazil

^[2] Univ Valencia, Fac CC Biol, Dept Genet, E-46100 Valencia - Spain

• Tipo de documento: Artigo Científico
• Fonte: Journal of Invertebrate Pathology; v. 127, p. 32-34, MAY 2015.
• Citações Web of Science: 5

Resumo

Sugarcane borer (Diatraea saccharalis, F.) is an important corn pest in South America and United States. The aim of the present study was to analyze the susceptibility and binding interactions of three Cry1A proteins and Cry1Fa in a Brazilian D. saccharalis population. The results showed that Cry1Ab was the most active, followed by Cry1Ac,Cry1Fa and Cry1Aa. All Cry1-biotinylated proteins tested bound specifically to the D. saccharalis brush border membrane vesicles (BBMV). Heterologous competition assays showed shared binding sites for all Cry1A proteins and another one shared by Cry1Fa and Cry1Ab. Thus, pyramiding Cry1Aa/Cry1Ac and Cry1F proteins would be a recommended strategy for managing this pest. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 09/18544-1 - Efeitos da Interação e Toxicidade das Proteínas Vip3Aa e Cry1Ac de Bacillus thuringiensis em populações de Diatraea saccharalis (Fabr., 1794) (LEPIDOPTERA:PYRALIDAE)
• Beneficiário: Camila Chiaradia Davolos
• Modalidade de apoio: Bolsas no Brasil - Doutorado