Phylogenetic and phylogeographic mapping of the avian coronavirus spike protein-encoding gene in wild and synanthropic birds

Autor(es)

Duraes-Carvalho, Ricardo ^{[1, 2, 3]} ; Caserta, Leonardo C. ^[1] ; Barnabe, Ana C. S. ^[1] ; Martini, Matheus C. ^[1] ; Simas, Paulo V. M. ^[1] ; Santos, Marcia M. B. ^[4] ; Salemi, Marco ^{[2, 3]} ; Arns, Clarice W. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Virol Lab, Dept Genet Evolut & Bioagents, BR-13083970 Campinas, SP - Brazil

^[2] Univ Florida, Emerging Pathogens Inst, Gainesville, FL - USA

^[3] Univ Florida, Dept Pathol Immunol & Lab Med, Gainesville, FL - USA

^[4] Univ Fed Juiz de Fora, Dept Biol Sci, Juiz De Fora, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIRUS RESEARCH; v. 201, p. 101-112, APR 2 2015.
• Citações Web of Science: 4

Resumo

The evolution and population dynamics of avian coronaviruses (AvCoVs) remain underexplored. In the present study, in-depth phylogenetic and Bayesian phylogeographic studies were conducted to investigate the evolutionary dynamics of AvCoVs detected in wild and synanthropic birds. A total of 500 samples, including tracheal and cloacal swabs collected from 312 wild birds belonging to 42 species, were analysed using molecular assays. A total of 65 samples (13%) from 22 bird species were positive for AvCoV. Molecular evolution analyses revealed that the sequences from samples collected in Brazil did not cluster with any of the AvCoV S1 gene sequences deposited in the GenBank database. Bayesian framework analysis estimated an AvCoV strain from Sweden (1999) as the most recent common ancestor of the AvCoVs detected in this study. Furthermore, the analysis inferred an increase in the AvCoV dynamic demographic population in different wild and synanthropic bird species, suggesting that birds may be potential new hosts responsible for spreading this virus. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/03922-6 - Investigação e caracterização filogenética de Coronavírus na biota de aves silvestres e sinantrópicas provenientes das Regiões Sul e Sudeste do Brasil
• Beneficiário: Ricardo Durães de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50919-5 - Investigação de agentes virais na biota de aves silvestres no estado de São Paulo
• Beneficiário: Clarice Weis Arns
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular