Efficient duplex solid-phase fluorescent assay (dFISA) for the simultaneous detection of specific anti-T. gondii IgG and IgM due to refined conjugates

Autor(es)

Rodrigues, J. P. ^[1] ; Andrade, Jr., H. F. ^[2]

Afiliação do(s) autor(es)

^[1] HCFMUSP, Inst Lab Invest Med, Sao Paulo - Brazil

^[2] Inst Trop Med S Paulo, Protozool Lab, BR-05403000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF IMMUNOLOGICAL METHODS; v. 420, p. 11-17, MAY 2015.
• Citações Web of Science: 0

Resumo

Toxoplasma gondii infections are very common, causing occasional central nervous system and eye diseases, and must be screened in prenatal care for efficient therapy. Here, we developed a duplex solid-phase fluorescent assay (dFISA) for the simultaneous detection of anti-T. gondii IgG and IgM antibodies in prenatal care screening for toxoplasmosis. Assays using commercially available ion-exchange purified conjugates yielded poor results and high background fluorescence. Same-well IgG/IgM dFISA with refined conjugates was used to test 140 samples from university students, 120 samples from pregnant women and 24 samples from adult volunteers at a large public hospital. We found that dFISA offers high concordance, specificity and reproducibility for IgG (Kappa = 0.883) and IgM (Kappa = 0.918), which is useful in high-throughput applications for antenatal care. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/04676-9 - Citometria de fluxo e fluorimetria em doenças causadas por protozoários
• Beneficiário: Heitor Franco de Andrade Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular