Antimicrobial resistance and virulence gene profiles in P. multocida strains isolated from cats

Autor(es)

Porfida Ferreira, Thais Sebastiana ^[1] ; Felizardo, Maria Roberta ^[1] ; Sena de Gobbi, Debora Dirani ^[1] ; Moreno, Marina ^[1] ; Moreno, Andrea Micke ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Lab Epidemiol Mol & Resistencia Antimicrobianos, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 46, n. 1, p. 271-277, MAY 2015.
• Citações Web of Science: 2

Resumo

Cats are often described as carriers of Pasteurella multocida in their oral microbiota. This agent is thought to cause pneumonia, conjunctivitis, rhinitis, gingivostomatitis, abscess and osteonecrosis in cats. Human infection with P. multocida has been described in several cases affecting cat owners or after cat bites. In Brazil, the cat population is approximately 21 million animals and is increasing, but there are no studies of the presence of P. multocida in the feline population or of human cases of infection associated with cats. In this study, one hundred and ninety-one healthy cats from owners and shelters in São Paulo State, Brazil, were evaluated for the presence of P. multocida in their oral cavities. Twenty animals were positive for P. multocida, and forty-one strains were selected and characterized by means of biochemical tests and PCR. The P. multocida strains were tested for capsular type, virulence genes and resistance profile. A total of 75.6% (31/41) of isolates belonged to capsular type A, and 24.4% (10/41) of the isolates were untypeable. None of the strains harboured toxA, tbpA or pfhA genes. The frequencies of the other genes tested were variable, and the data generated were used to build a dendrogram showing the relatedness of strains, which were clustered according to origin. The most common resistance profile observed was against sulfizoxazole and trimethoprim-sulphamethoxazole. (AU)

• Processo FAPESP: 07/08592-3 - Isolamento e caracterização de Pasteurella multocida provenientes de cães e gatos.
• Beneficiário: Thaís Sebastiana Porfida Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/03024-7 - Avaliação do perfil sorológico e de eliminação de Pasteurella multocida em rebanhos suínos
• Beneficiário: Andrea Micke Moreno
• Modalidade de apoio: Auxílio à Pesquisa - Regular