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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Anti-inflammatory sesquiterpene lactones from Tithonia diversifolia trigger different effects on human neutrophils

Texto completo
Autor(es):
Abe, Aneli E. [1] ; de Oliveira, Carine E. [1] ; Dalboni, Thalita M. [1] ; Chagas-Paula, Daniela A. [2] ; Rocha, Bruno A. [2] ; de Oliveira, Rejane B. [2] ; Gasparoto, Thais H. [1] ; da Costa, Fernando B. [2] ; Campanelli, Ana P. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Biol Sci, Bauru, SP - Brazil
[2] Univ Sao Paulo, AsterBioChem Res Team, Sch Pharmaceut Sci Ribeirao Preto, Lab Pharmacognosy, Dept Pharmaceut Sci, BR-14049 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 25, n. 2, p. 111-116, MAR-APR 2015.
Citações Web of Science: 11
Resumo

The tagitinins isolated of Tithonia diversifolia (Hemsl.) A. Gray, Asteraceae, are the most studied sesquiterpene lactones due to their wide spectrum of pharmacologic activities, especially related with nuclear factor-kappa B inhibition. Nevertheless, detailed studies about the mechanism of action of its activecompounds are still lacking. Neutrophils perform a fundamental role in the inflammatory response to several etiologic factors. However, the effect of tagitinins on human neutrophil is not yet clearly known. We investigated the role of tagitinin C (1), tagitinin F (2) and tagitinin A (3) in activation and survival of human neutrophils to establish possible effects in their mechanisms of inflammation. Human neutrophils were purified from the peripheral blood and cultivated with tagitinins C (1), F (2) and A (3) in the presence or not of Escherichia coli lipopolysaccharide. The enzymatic activity, apoptosis and secretion of cytokines rate were determined after 18 h. Lipopolysaccharide-induced myeloperoxidase activity of human neutrophils was significantly inhibited only by tagitinin F (2). Apoptosis of neutrophils was increased in the presence of tagitinin C (1), and it occurred independently of the presence of lipopolysaccharide or dexamethasone. Tagitinins C (1), F (2) and A (3) decrease lipopolysaccharide-induced interleukin-6, interleukin-8 and Tumor necrosis factor alpha production by human neutrophils. Together, these results indicate that tagitinins exhibit anti-inflammatory action on human neutrophils. However, tagitinin F (2) was the only sesquiterpene lactone that decreased secretion of inflammatory products by neutrophils without inducing neutrophil apoptosis. (C) 2015 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved. (AU)

Processo FAPESP: 08/57149-8 - Potencial químico e farmacológico de espécies da familia Asteraceae: estudos integrados in vivo, in vitro e in silico
Beneficiário:Fernando Batista da Costa
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/18174-0 - Estudo da atividade imuno-modulatória de micromoléculas isoladas de extratos de Tithonia diversifolia em neutrófilos humanos
Beneficiário:Aneli Eiko Abe
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 10/19317-6 - Perfil de ativação de neutrófilos de idosos e expressão de receptores de IgG
Beneficiário:Thalita Marcato Dalboni
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 09/14127-7 - O papel do inflamassoma nos tumores quimicamente induzidos
Beneficiário:Thaís Helena Gasparoto
Linha de fomento: Bolsas no Brasil - Pós-Doutorado