ETHANOL PRECIPITATION OF GLYCOSYL HYDROLASES PRODUCED BY Trichoderma harzianum P49P11

Autor(es)

Marino, M. A. ^{[1, 2]} ; Freitas, S. ^[2] ; Miranda, E. A. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Chem Engn, Dept Mat & Bioproc Engn, UNICAMP, FEQ, BR-13083852 Campinas, SP - Brazil

^[2] Brazilian Ctr Res Energy & Mat, Brazilian Bioethanol Sci & Technol Lab CTBE, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Chemical Engineering; v. 32, n. 2, p. 325-333, APR-JUN 2015.
• Citações Web of Science: 3

Resumo

AbstractThis study aimed to concentrate glycosyl hydrolases produced by Trichoderma harzianum P49P11 by ethanol precipitation. The variables tested besides ethanol concentration were temperature and pH. The precipitation with 90% (v/v) ethanol at pH 5.0 recovered more than 98% of the xylanase activity, regard less of the temperature (5.0, 15.0, or 25.0 °C). The maximum recovery of cellulase activity as FPase was 77% by precipitation carried out at this same pH and ethanol concentration but at 5.0 °C. Therefore, ethanol precipitation can be considered to be an efficient technique for xylanase concentration and, to a certain extent, also for the cellulase complex. (AU)

• Processo FAPESP: 10/08089-2 - Desenvolvimento de uma plataforma de expressão, baseada em E. coli, para a produção de enzimas envolvidas na hidrólise de biomassas lignocelulósicas
• Beneficiário: Sindelia Freitas Azzoni
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores