| Full text | |
| Author(s): |
Total Authors: 3
|
| Affiliation: | [1] Univ Estadual Campinas, Sch Chem Engn, Dept Mat & Bioproc Engn, UNICAMP, FEQ, BR-13083852 Campinas, SP - Brazil
[2] Brazilian Ctr Res Energy & Mat, Brazilian Bioethanol Sci & Technol Lab CTBE, Campinas, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Chemical Engineering; v. 32, n. 2, p. 325-333, APR-JUN 2015. |
| Web of Science Citations: | 3 |
| Abstract | |
AbstractThis study aimed to concentrate glycosyl hydrolases produced by Trichoderma harzianum P49P11 by ethanol precipitation. The variables tested besides ethanol concentration were temperature and pH. The precipitation with 90% (v/v) ethanol at pH 5.0 recovered more than 98% of the xylanase activity, regard less of the temperature (5.0, 15.0, or 25.0 °C). The maximum recovery of cellulase activity as FPase was 77% by precipitation carried out at this same pH and ethanol concentration but at 5.0 °C. Therefore, ethanol precipitation can be considered to be an efficient technique for xylanase concentration and, to a certain extent, also for the cellulase complex. (AU) | |
| FAPESP's process: | 10/08089-2 - Development of an expression platform, based on E. coli, for the production of enzymes used for lignocellulosic biomass hydrolysis |
| Grantee: | Sindelia Freitas Azzoni |
| Support Opportunities: | Research Grants - Young Investigators Grants |