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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E

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Autor(es):
Santana, Andreia T. [1] ; Guelfi, Marieli [1] ; Medeiros, Hyllana C. D. [1] ; Tavares, Marco A. [1] ; Bizerra, Paulo F. V. [1] ; Mingatto, Fabio E. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Lab Metab & Toxicol Biochem, BR-17900000 Dracena, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Biological Research; v. 48, JUL 31 2015.
Citações Web of Science: 12
Resumo

BACKGROUND: Gossypol is a chemical present in the seeds of cotton plants (Gossypium sp.) that reduces fertility in farm animals. Vitamin E is an antioxidant and may help to protect cells and tissues against the deleterious effects of free radicals. The aim of this study was to evaluate the mechanisms of reproductive toxicity of gossypol in rats and the protective effects of vitamin E. Forty Wistar rats were used, divided into four experimental groups (n = 10): DMSO/ saline + corn oil; DMSO/saline + vitamin E; gossypol + corn oil; and gossypol + vitamin E. RESULTS: Fertility was significantly reduced in male rats treated with gossypol in that a significant decrease in epididy-mal sperm count was observed (P < 0.05) and the number of offspring was significantly reduced in females mated with them (P < 0.05). This dysfunction was prevented by vitamin E. Gossypol caused a significant increase in the activity of the enzymes glutathione peroxidase (P < 0.01) and glutathione reductase (P < 0.01), but vitamin E did not reduce the enzyme activities (P > 0.05). The levels of reduced glutathione and pyridine nucleotides in testis homogen-ate were significantly reduced by gossypol (P < 0.05 and P < 0.01, respectively) and this reduction was accompanied by increased levels of oxidized glutathione (P < 0.05). Vitamin E showed a preventive effect on the changes in the levels of these substances. Gossypol significantly increased the levels of malondialdehyde (P < 0.01), a lipid peroxida-tion indicator, whereas treatment with vitamin E inhibited the action of the gossypol. Vitamin E prevented a decrease in mitochondrial ATP induced by gossypol (P < 0.05). CONCLUSIONS: This study suggests that the reproductive dysfunction caused by gossypol may be related to oxidative stress and mitochondrial bioenergetic damage and that treatment with vitamin E can prevent the infertility caused by the toxin. (AU)

Processo FAPESP: 12/08668-8 - Mecanismos da infertilidade causada por gossipol em ratos e efeito protetor da vitamina e
Beneficiário:Andréia Tieme de Santana
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 12/22684-6 - Mecanismos da infertilidade causada por gossipol em ratos e efeito protetor da vitamina E
Beneficiário:Fábio Erminio Mingatto
Modalidade de apoio: Auxílio à Pesquisa - Regular