Isolation and enzyme bioprospection of endophytic bacteria associated with plants of Brazilian mangrove ecosystem

Autor(es)

Castro, Renata A. ^[1] ; Quecine, Maria Carolina ^[2] ; Lacava, Paulo T. ^[3] ; Batista, Bruna D. ^[2] ; Luvizotto, Danice M. ^[2] ; Marcon, Joelma ^[2] ; Ferreira, Anderson ^[4] ; Melo, Itamar S. ^[5] ; Azevedo, Joao L. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr CENA, Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz ESALQ, Dept Genet, Piracicaba, SP - Brazil

^[3] Fed Univ Sao Carlos UFSCar, Dept Morphol & Pathol, Ctr Biol & Hlth Sci, Sao Carlos, SP - Brazil

^[4] Brazilian Agr Res Corp Embrapa Agrosilvopastoral, Sinop, MS - Brazil

^[5] CNPMA Embrapa Environm, Lab Environm Microbiol, Jaguariuna, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SPRINGERPLUS; v. 3, JUL 28 2014.
• Citações Web of Science: 25

Resumo

The mangrove ecosystem is a coastal tropical biome located in the transition zone between land and sea that is characterized by periodic flooding, which confers unique and specific environmental conditions on this biome. In these ecosystems, the vegetation is dominated by a particular group of plant species that provide a unique environment harboring diverse groups of microorganisms, including the endophytic microorganisms that are the focus of this study. Because of their intimate association with plants, endophytic microorganisms could be explored for biotechnologically significant products, such as enzymes, proteins, antibiotics and others. Here, we isolated endophytic microorganisms from two mangrove species, Rhizophora mangle and Avicennia nitida, that are found in streams in two mangrove systems in Bertioga and Cananeia, Brazil. Bacillus was the most frequently isolated genus, comprising 42% of the species isolated from Cananeia and 28% of the species from Bertioga. However, other common endophytic genera such as Pantoea, Curtobacterium and Enterobacter were also found. After identifying the isolates, the bacterial communities were evaluated for enzyme production. Protease activity was observed in 75% of the isolates, while endoglucanase activity occurred in 62% of the isolates. Bacillus showed the highest activity rates for amylase and esterase and endoglucanase. To our knowledge, this is the first reported diversity analysis performed on endophytic bacteria obtained from the branches of mangrove trees and the first overview of the specific enzymes produced by different bacterial genera. This work contributes to our knowledge of the microorganisms and enzymes present in mangrove ecosystems. (AU)

• Processo FAPESP: 10/50445-0 - Estado da variabilidade genética de Puccinia psidii Winter, agente causal de ferrugem em Eucalyptus spp.
• Beneficiário: Maria Carolina Quecine Verdi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/18740-5 - Detecção e Caracterização de Quitinases em bibliotecas metagenomicas de Manguezais do Estado de São Paulo.
• Beneficiário: Joelma Marcon
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/05204-8 - Promoção de crescimento vegetal por rizobactérias do guaranazeiro
• Beneficiário: Bruna Durante Batista
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/13910-6 - Biodiversidade e atividades funcionais de microrganismos de manguezais do estado de São Paulo
• Beneficiário: Itamar Soares de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático