Short-term PTH administration increases dentine apposition and microhardness in mice

Autor(es)

Guimaraes, Gustavo Narvaes ^[1] ; Camargo Cardoso, Guinea Brasil ^[2] ; Naves, Lucas Zago ^[3] ; Correr-Sobrinho, Lourenco ^[3] ; Peres Line, Sergio Roberto ^[1] ; Marques, Marcelo Rocha ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Morphol, Div Histol, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Fac Mech Engn, Dept Mat Engn, BR-13414903 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dent Mat Div, Dept Restorat Dent, BR-13414903 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 57, n. 10, p. 1313-1319, OCT 2012.
• Citações Web of Science: 4

Resumo

Objective: The purpose of this study is to investigate the effects of intermittent parathyroid hormone (PTH) administration on the apposition rate and structural features of dentine from mouse incisors. Methods: Young male A/J Unib mice were treated daily for 6 and 10 days with 40 mu g/kg of hPTH 1-34 or a vehicle. Dentine apposition rates measured by fluorescent labels (tetracycline and calcein) and alkaline phosphatase (ALP) plasma levels were evaluated after 6 days of treatment. Knoop microhardness testing and element content measurements in at.% of calcium (Ca), phosphorus (P), oxygen (0), and magnesium (Mg) in the peritubular and intertubular dentine were performed by Energy Dispersive X-ray (EDX) microanalysis via Scanning Electron Microscopy (SEM) after 10 days of treatment. Results: Histometric analysis revealed an increase of 5% in the apposition rate of dentine and 25% in the ALP plasma levels in the PTH treated group. In addition, knoop microhardness testing revealed that the animals treated with PTH had a greater microhardness (11%). EDX microanalysis showed that PTH treatment led to increases in P (23%) and Ca (53%) at.% content, as well as the Ca/P ratio (24%) in peritubular dentine. The chemical composition of intertubular dentine did not vary between the groups. Conclusions: These findings indicate that intermittent administration of hPTH (1-34) increases apposition and mineralization of the dentine during young mice incisor formation. (c) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/06125-4 - Investigação do efeito da administração intermitente do hormônio paratireóideo na formação de dentina e em células de linhagem odontoblástica
• Beneficiário: Marcelo Rocha Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular