Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Short-term PTH administration increases dentine apposition and microhardness in mice

Full text
Author(s):
Guimaraes, Gustavo Narvaes [1] ; Camargo Cardoso, Guinea Brasil [2] ; Naves, Lucas Zago [3] ; Correr-Sobrinho, Lourenco [3] ; Peres Line, Sergio Roberto [1] ; Marques, Marcelo Rocha [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Morphol, Div Histol, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Estadual Campinas, Fac Mech Engn, Dept Mat Engn, BR-13414903 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dent Mat Div, Dept Restorat Dent, BR-13414903 Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 57, n. 10, p. 1313-1319, OCT 2012.
Web of Science Citations: 4
Abstract

Objective: The purpose of this study is to investigate the effects of intermittent parathyroid hormone (PTH) administration on the apposition rate and structural features of dentine from mouse incisors. Methods: Young male A/J Unib mice were treated daily for 6 and 10 days with 40 mu g/kg of hPTH 1-34 or a vehicle. Dentine apposition rates measured by fluorescent labels (tetracycline and calcein) and alkaline phosphatase (ALP) plasma levels were evaluated after 6 days of treatment. Knoop microhardness testing and element content measurements in at.% of calcium (Ca), phosphorus (P), oxygen (0), and magnesium (Mg) in the peritubular and intertubular dentine were performed by Energy Dispersive X-ray (EDX) microanalysis via Scanning Electron Microscopy (SEM) after 10 days of treatment. Results: Histometric analysis revealed an increase of 5% in the apposition rate of dentine and 25% in the ALP plasma levels in the PTH treated group. In addition, knoop microhardness testing revealed that the animals treated with PTH had a greater microhardness (11%). EDX microanalysis showed that PTH treatment led to increases in P (23%) and Ca (53%) at.% content, as well as the Ca/P ratio (24%) in peritubular dentine. The chemical composition of intertubular dentine did not vary between the groups. Conclusions: These findings indicate that intermittent administration of hPTH (1-34) increases apposition and mineralization of the dentine during young mice incisor formation. (c) 2012 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 09/06125-4 - Evaluation of the effect of intermittent PTH administration on odontoblast-like cells and dentine formation
Grantee:Marcelo Rocha Marques
Support Opportunities: Regular Research Grants