Evaluation of the effect of intermittent PTH administration on odontoblast-like cells and dentine formation

• Grant number: 09/06125-4
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2009 - October 31, 2011
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Marcelo Rocha Marques
• Grantee: Marcelo Rocha Marques
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Parathyroid hormone (PTH) is an important calcium metabolism regulator and is known to induce bone anabolism in humans and rodents, when intermittently administered. The bone anabolism caused by PTH administration, can treat osteoporosis and to accelerate bone healing. Although most studies investigating PTH functions involve bone cells, other cells such as odontoblast, that also produce mineralized tissue (dentine), have been reported as having ability to express PTH receptor. This study is aimed at investigating the effect of intermittent hPTH(1-34) administration on gene expression (Col1a1: collagen, type I, alpha 1; Dmp1: dentin matrix protein 1; Dspp: dentin sialophosphoprotein; Alpl: alkaline phosphatase; Pth1r: parathyroid hormone 1 receptor, apoptosis and proliferation rates and alkaline phosphatase (ALP) activity of osteoblastic linage cells (MDPC-23). The effect of intermittent PTH administration on dentine formation will also be evaluated. Incisors, continuously growing teeth, will be used to investigate the effect of PTH on dentine apposition rate in mice, by using fluorescent markings. The results will be evaluated by statistical tests, and values at 5% level of significance will be considered. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUIMARAES, GUSTAVO NARVAES; CAMARGO CARDOSO, GUINEA BRASIL; NAVES, LUCAS ZAGO; CORRER-SOBRINHO, LOURENCO; PERES LINE, SERGIO ROBERTO; MARQUES, MARCELO ROCHA. Short-term PTH administration increases dentine apposition and microhardness in mice. ARCHIVES OF ORAL BIOLOGY, v. 57, n. 10, p. 1313-1319, OCT 2012. (09/06125-4)

GUIMARAES, GUSTAVO NARVAES; STIPP, RAFAEL NOBREGA; RODRIGUES, THAISANGELA LOPES; DE SOUZA, ANA PAULA; PERES LINE, SERGIO ROBERTO; MARQUES, MARCELO ROCHA. Evaluation of the effects of transient or continuous PTH administration to odontoblast-like cells. ARCHIVES OF ORAL BIOLOGY, v. 58, n. 6, p. 638-645, JUN 2013. (09/06125-4)

GUIMARAES, GUSTAVO NARVAES; RODRIGUES, THAISANGELA LOPES; DE SOUZA, ANA PAULA; LINE, SERGIO ROBERTO; MARQUES, MARCELO ROCHA. Parathyroid Hormone (1-34) Modulates Odontoblast Proliferation and Apoptosis via PKA and PKC-Dependent Pathways. Calcified Tissue International, v. 95, n. 3, p. 275-281, SEP 2014. (09/06125-4)