Evaluation of the effects of transient or continuous PTH administration to odontoblast-like cells

Autor(es)

Guimaraes, Gustavo Narvaes ^[1] ; Stipp, Rafael Nobrega ^[2] ; Rodrigues, Thaisangela Lopes ^[3] ; de Souza, Ana Paula ^[1] ; Peres Line, Sergio Roberto ^[1] ; Marques, Marcelo Rocha ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Morphol, Div Histol, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Oral Diag, Campinas, SP - Brazil

^[3] Univ Fed Goias, Sch Dent, Dept Stomatol Sci, Goiania, Go - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 58, n. 6, p. 638-645, JUN 2013.
• Citações Web of Science: 2

Resumo

Parathyroid hormone participates in the metabolism of mineralized tissue. Its role in the formation of dentine is, as yet, incompletely understood. In the present study we analyzed the effect of transient (1 and 24-h/cycle) or continuous hPTH (1-34) treatment in odontoblast-like cells (MDPC-23) to the following parameters: mineral deposition detected by alizarin red, mRNA expression of the type I collagen (COL1), alkaline phosphatase (ALP), biglycan (BGN), matrix metalloproteinase 2 (MMP-2) and dentine sialophosphoprotein (DSPP) quantified by qRT-PCR. MMP-2 and ALP activities were quantified by zymography and colorimetric assay, respectively. The results showed that compared to Control group: intermittent PTH administration (1 and 24-h/cycle) decreased the mineral deposition and ALP activity. DSPP gene expression was not detected in both control and PTH treated cells. The PTH administration for 24-h/cycle increased the ALP, BGN and COL1 mRNA expression and continuous PTH treatment increased BGN and COL1 mRNA expression. Zymography assays showed that compared to Control group: PTH treatment for 1-h/cycle increased the total MMP-2 secretion and the continuous treatment decreased the secreted levels of MMP-2 active-form. Taken together, the results shown that PTH may regulate the odontoblast-like cells-induced secretion, and potentially this hormone can affect in vivo odontoblasts functions. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/06125-4 - Investigação do efeito da administração intermitente do hormônio paratireóideo na formação de dentina e em células de linhagem odontoblástica
• Beneficiário: Marcelo Rocha Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular