Torque teno sus virus 1 and 2 viral loads in faeces of porcine circovirus 2-positive pigs

Autor(es)

de Castro, Alessandra M. M. G. ^{[1, 2]} ; Baldin, Cintia M. ^[1] ; Favero, Cintia M. ^[1] ; Gerber, Priscilla F. ^[3] ; Cipullo, Rafael I. ^[1] ; Richtzenhain, Leonardo J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Vet Med, Dept Prevent Vet Med & Anim Hlth, Sao Paulo - Brazil

^[2] Fac Med Vet, Complexo Educ Fac Metropolitanas Unidas, Sao Paulo, SP - Brazil

^[3] Univ Fed Minas Gerais, Dept Pathol, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA VETERINARIA BRNO; v. 84, n. 2, p. 91-95, 2015.
• Citações Web of Science: 1

Resumo

Recently, studies have suggested an association between the Torque teno sus virus (TTSuV) and the Porcine circovirus-2 (PCV2) in PCV2-associated disease cases. The aim of this study was to verify TTSuVs loads in pig faeces from PCV2-positive animals with and without diarrhea from PCVAD-affected and PCV2-unvaccinated herds. A total of 80 faecal samples were collected individually from nursery and grow-finish pigs with (n = 40) or without (n = 40) diarrhea. The samples were tested for PCV2 and TTSuVs by using DNA binding dye SYBR Green quantitative polymerase chain reaction (qPCR). Torque teno sus virus k2 (TTSuVk2) load in the faeces was significantly higher in the nursery pigs with diarrhea, and these pigs also exhibited significantly higher PCV2 (P < 0.01) faecal matter loads compared to the non-diarrheic animals from the same age group. Torque teno sus virus I (TTSuV1) viral loads were the same regardless of age group and disease condition. There were no correlations between PCV2 and TTSuV1 or TTSuVk2 and TTSuV1 viral loads; however, a weak correlation (r = 0.23, P = 0.03) was found between TTSuVk2 and PCV2 viral loads. In conclusion, ITSuVk2 viral loads were significantly higher in the diarrheic faeces from the nursery pigs. Additionally, the higher loads of PCV2 and TTSuVk2 in the nursery-diarrheic animals revealed that diarrhea might have an important role in the spread of both viruses in herds. (AU)

• Processo FAPESP: 07/57115-3 - Circovirus suíno tipo 2 (PCV-2): patogenia comparada dos subtipos A e B e efeitos sobre o desempenho zootécnico de suínos experimentalmente infectados com amostras brasileiras
• Beneficiário: Alessandra Marnie Martins Gomes de Castro
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 08/58822-8 - Circovirus suino tipo 2 (pcv2): deteccao em fezes oriundas de suinos de granjas positivas para o agente.
• Beneficiário: Cintia Manzatto Baldin
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica