Direct coupling of a free-flow isotachophoresis (FFITP) device with electrospray ionization mass spectrometry (ESI- MS)

Autor(es)

Park, J. K. ^[1] ; Campos, C. D. M. ^{[2, 1]} ; Neuzil, P. ^{[3, 1]} ; Abelmann, L. ^{[1, 4]} ; Guijt, R. M. ^{[1, 5, 6]} ; Manz, A. ^[1]

Afiliação do(s) autor(es)

^[1] Korea Inst Sci & Technol KIST Europe, D-66123 Saarbrucken - Germany

^[2] Univ Estadual Campinas, BR-13083970 Campinas, SP - Brazil

^[3] Brno Univ Technol, Cent European Inst Technol, CZ-61600 Brno - Czech Republic

^[4] Univ Twente, MESA, NL-7500 AE Enschede - Netherlands

^[5] Univ Tasmania, Sch Med, Hobart, Tas 7001 - Australia

^[6] Univ Tasmania, ACROSS, Hobart, Tas 7001 - Australia

• Tipo de documento: Artigo Científico
• Fonte: LAB ON A CHIP; v. 15, n. 17, p. 3495-3502, 2015.
• Citações Web of Science: 10

Resumo

We present the online coupling of a free-flow isotachophoresis (FFITP) device to an electrospray ionization mass spectrometer (ESI-MS) for continuous analysis without extensive sample preparation. Free-flow-electrophoresis techniques are used for continuous electrophoretic separations using an electric field applied perpendicular to the buffer and sample flow, with FFITP using a discontinuous electrolyte system to concurrently focus a target analyte and remove interferences. The online coupling of FFITP to ESI-MS decouples the separation and detection timeframe because the electrophoretic separation takes place perpendicular to the flow direction, which can be beneficial for monitoring (bio)chemical changes and/or extensive MSn studies. We demonstrated the coupling of FFITP with ESI-MS for simultaneous concentration of target analytes and sample clean-up. Furthermore, we show hydrodynamic control of the fluidic fraction injected into the MS, allowing for fluidically controlled scanning of the ITP window. Future applications of this approach are expected in monitoring biochemical changes and proteomics. (AU)

• Processo FAPESP: 13/06625-2 - Desenvolvimento de microdispositivos enzimáticos para avaliação de glutamato em matrizes complexas
• Beneficiário: Camila Dalben Madeira Campos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/02477-3 - Desenvolvimento de um Lab-on-a-chip para análise de glutamato em alimentos
• Beneficiário: Camila Dalben Madeira Campos
• Modalidade de apoio: Bolsas no Brasil - Doutorado