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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Possible Involvement of IL-21 and IL-10 on Salivary IgA Levels in Chronic Periodontitis Subjects

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Autor(es):
Napimoga, M. H. [1] ; Nunes, L. H. A. C. [1] ; Maciel, A. A. B. [1] ; Demasi, A. P. D. [1] ; Benatti, B. B. [2] ; Santos, V. R. [3] ; Bastos, M. F. [3] ; de Miranda, T. S. [3] ; Duarte, P. M. [3]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Sao Leopoldo Mand Inst & Res Ctr, Lab Immunol & Mol Biol, Campinas, SP - Brazil
[2] Univ Fed Maranhao, Sch Dent, Sao Luis, MA - Brazil
[3] Univ Guarulhos, Dept Periodont, Dent Res Div, Guarulhos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Scandinavian Journal of Immunology; v. 74, n. 6, p. 596-602, DEC 2011.
Citações Web of Science: 9
Resumo

Specific cytokines and the costimulatory protein CD40 play role in inducing immunoglobulin (Ig)A production by B cells in the humoral immune response. However, to date, the role of these mediators was not investigated in chronic periodontitis. Therefore, the aim of this study was to assess the local levels of interleukin (IL)-21, IL-21 receptor (IL-21R), IL-4, IL-10 and CD40 ligand (CD40L) on chronic periodontitis subjects and their relationship with the salivary levels of IgA. Gingival biopsies and un-stimulated saliva were collected from chronic periodontitis (n = 15) and periodontally healthy (n = 15) subjects. The mRNA levels of IL-4, IL-10, IL-21, IL-21R, CD40L in the gingival biopsies were evaluated by quantitative real-time polymerase chain reaction. The salivary levels of IgA and the levels of IL-4 and IL-10 in the gingival biopsies were analyzed by ELISA. The mean levels of IgA were significantly higher in the chronic periodontitis compared to periodontally healthy group (P < 0.05). The mRNA levels for IL-21 was higher (P < 0.05) in the chronic periodontitis when compared to the healthy group. However, the expression of IL-21R and CD40L did not differ between groups. The IL-10 was significantly elevated at mRNA and protein levels in chronic periodontitis when compared to periodontally healthy group (P < 0.05). Conversely, the mRNA levels as well as the protein amount of IL-4 were significantly lower (P < 0.05) in chronic periodontitis than healthy ones. In conclusion, the upregulation of IL-21 and IL-10 and downregulation of IL-4 in periodontitis tissues may be collectively involved in the increased levels of salivary IgA in chronic periodontitis subjects. (AU)

Processo FAPESP: 08/09687-0 - Tratamento periodontal em indivíduos diabéticos compensados e não-compensados portadores de Diabetes Mellitus: avaliação clínica, glicêmica e dos níveis de citocinas e fatores de reabsorção óssea
Beneficiário:Poliana Mendes Duarte
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/04280-0 - Expressão gênica em sítios com periodontite crônica em indivíduos portadores de Diabetes Mellitus
Beneficiário:Poliana Mendes Duarte
Linha de fomento: Auxílio à Pesquisa - Regular