Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine

Autor(es)

Silva, Miguel Ladino ^{[1, 2]} ; Ribeiro, Alexandre Pinto ^[3] ; Silva, Germana Alegro ^[1] ; Barbosa Sanchez, Irma Ximena ^[4] ; Renzo, Roberta ^[1] ; Uscategui, Ricardo ^[1] ; Lima, Tiago Barbalho ^[1] ; Aldrovani, Marcela ^[1] ; Laus, Jose Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Med & Surg, Coll Agr & Vet Sci, Jaboticabal, SP - Brazil

^[2] La Salle Univ, Coll Agr Sci, Bogota - Colombia

^[3] Univ Fed Mato Grosso, Coll Vet & Agr Sci, Dept Vet Clin Med, Cuiaba, MT - Brazil

^[4] Univ Tolima, Coll Vet Med & Anim Sci, Ibague - Colombia

• Tipo de documento: Artigo Científico
• Fonte: Arquivos Brasileiros de Oftalmologia; v. 78, n. 3, p. 141-145, MAY-JUN 2015.
• Citações Web of Science: 1

Resumo

Purposes: To evaluate the effects of nalbuphine 1% on the expression of metalloproteinase 1 (MMP-1), metalloproteinase 9 (MMP-9), and opioid growth factor (OGF) in rabbit corneas after lamellar keratectomy. Methods: The rabbits were assigned to two groups: group nalbuphine (GN, n=30), which received 30 mu L of nalbuphine 1% in 4 daily applications at regular intervals until corneal epithelialization, and group control (GC, n=30), which received physiological saline solution under the same conditions adopted in GN. The corneas were collected for immunohistochemistry on days 1, 3, 5, 7, and 9 after lamellar keratectomy, and the expressions of MMP-1, MMP-9, and OGF were analyzed. Results: The expressions of MMP-1 and MMP-9 increased until day 5 of the evaluation, with no differences observed between GN and GC (p>0.05). On days 7 and 9, significant reductions were observed in the expression of MMP-1 (p<0.01), with no differences observed between GN and GC (p>0.05). The expression of OGF was constant in all periods (p>0.05), restricted to the corneal epithelium, and there was no difference between the groups (p>0.05). Conclusions: The study results showed that nalbuphine 1% did not alter the expression patterns of MMP-1, MMP-9, and OGF in rabbit corneas after lamellar keratectomy. (AU)

• Processo FAPESP: 09/50082-8 - Expressao da metalo-protease-1, da metaloprotease-9 e do fator de crescimento opioide, em corneas de coelhos ceratectomizadas lamelarmente e tratadas com nalbufina a 1%.
• Beneficiário: José Luiz Laus
• Modalidade de apoio: Auxílio à Pesquisa - Regular