Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine

Full text
Author(s):
Silva, Miguel Ladino [1, 2] ; Ribeiro, Alexandre Pinto [3] ; Silva, Germana Alegro [1] ; Barbosa Sanchez, Irma Ximena [4] ; Renzo, Roberta [1] ; Uscategui, Ricardo [1] ; Lima, Tiago Barbalho [1] ; Aldrovani, Marcela [1] ; Laus, Jose Luiz [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dept Med & Surg, Coll Agr & Vet Sci, Jaboticabal, SP - Brazil
[2] La Salle Univ, Coll Agr Sci, Bogota - Colombia
[3] Univ Fed Mato Grosso, Coll Vet & Agr Sci, Dept Vet Clin Med, Cuiaba, MT - Brazil
[4] Univ Tolima, Coll Vet Med & Anim Sci, Ibague - Colombia
Total Affiliations: 4
Document type: Journal article
Source: Arquivos Brasileiros de Oftalmologia; v. 78, n. 3, p. 141-145, MAY-JUN 2015.
Web of Science Citations: 1
Abstract

Purposes: To evaluate the effects of nalbuphine 1% on the expression of metalloproteinase 1 (MMP-1), metalloproteinase 9 (MMP-9), and opioid growth factor (OGF) in rabbit corneas after lamellar keratectomy. Methods: The rabbits were assigned to two groups: group nalbuphine (GN, n=30), which received 30 mu L of nalbuphine 1% in 4 daily applications at regular intervals until corneal epithelialization, and group control (GC, n=30), which received physiological saline solution under the same conditions adopted in GN. The corneas were collected for immunohistochemistry on days 1, 3, 5, 7, and 9 after lamellar keratectomy, and the expressions of MMP-1, MMP-9, and OGF were analyzed. Results: The expressions of MMP-1 and MMP-9 increased until day 5 of the evaluation, with no differences observed between GN and GC (p>0.05). On days 7 and 9, significant reductions were observed in the expression of MMP-1 (p<0.01), with no differences observed between GN and GC (p>0.05). The expression of OGF was constant in all periods (p>0.05), restricted to the corneal epithelium, and there was no difference between the groups (p>0.05). Conclusions: The study results showed that nalbuphine 1% did not alter the expression patterns of MMP-1, MMP-9, and OGF in rabbit corneas after lamellar keratectomy. (AU)