The role of nicotine, cotinine and caffeine on the electrochemical behavior and bacterial colonization to cp-Ti

Autor(es):Mostrar menos -

Barao, Valentim A. R. ^[1] ; Ricomini-Filho, Antonio P. ^[2] ; Faverani, Leonardo P. ^[3] ; Del Bel Cury, Altair A. ^[1] ; Sukotjo, Cortino ; Monteiro, Douglas R. ^[3] ; Yuan, Judy Chia-Chun ^[4] ; Mathew, Mathew T. ^[5] ; do Amaral, Regiane C. ^[6] ; Mesquita, Marcelo E. ^[1] ; da Silva, Wander J. ^[1] ; Assuncao, Wirley G. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, BR-13414903 Sao Paulo - Brazil

^[2] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, BR-13414903 Sao Paulo - Brazil

^[3] Univ Estadual Paulista UNESP, Dept Dent Mat & Prosthodont, Aracatuba Dent Sch, BR-16015050 Sao Paulo - Brazil

^[4] Univ Illinois, Coll Dent, Dept Restorat Dent, Chicago, IL 60612 - USA

^[5] Rush Univ, Dept Orthoped Surg, Med Ctr, Chicago, IL 60612 - USA

^[6] Univ Campinas UNICAMP, Dept Publ Hlth, Piracicaba Dent Sch, BR-13414903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 56, p. 114-124, NOV 1 2015.
• Citações Web of Science: 17

Resumo

Although smoking promotes deleterious effect to bone healing, there is a lack of study investigating its role on the implant structure and biofilm growth. We hypothesized that nicotine, cotinine and caffeine would impair the corrosion resistance of commercially-pure titanium (cp-Ti) and would enhance Streptococcus sanguinis biofilm growth. Neither the smoking products nor the caffeine affected the corrosion tendency (P>.05) and the oxide layer resistance (P=.762) of cp-Ti. Lower capacitance values were noted in the presence of nicotine (P=.001) and cotinine (P=.0006). SEM showed no pitting corrosion, and the EDS spectra did not differ among groups. Nicotine (300 mu g/mL) induced higher surface roughness (P=.03) and greater surface change of cp-Ti. Nicotine at 3 mu g/ml, and cotinine at 03 and 3 mu g/mL increased the number of viable cells (P<.05). Biofilm exposed to nicotine (03,3 and 30 mu g/mL) (P=.025, .030, .040, respectively) and cotinine (3 and 30 mu g/mL) (P=.027, .049, respectively) enhanced carbohydrate content. Biofilm biomass and protein content were similar among groups (P>.05). These findings suggest a greater biofilm accumulation in smokers, a risk factor that may lead to peri-implantitis. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/20021-7 - O papel da nicotina, cotinina e cafeína no comportamento eletroquímico e colonização bacteriana do Ticp. um estudo in vitro
• Beneficiário: Wirley Goncalves Assuncao
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/20017-0 - O Papel da Nicotina, Cotinina e Cafeína no Comportamento Eletroquímico e Colonização Bacteriana do Ticp. Um estudo in vitro.
• Beneficiário: Valentim Adelino Ricardo Barão
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado