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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Functional characterization of two SOS-regulated genes involved in mitomycin C resistance in Caulobacter crescentus

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Autor(es):
Lopes-Kulishev, Carina O. [1] ; Alves, Ingrid R. [1] ; Valencia, Este La Y. [1] ; Pidhirnyj, Maria I. [1] ; Fernandez-Silva, Frank S. [1] ; Rodrigues, Ticiane R. [1] ; Guzzo, Cristiane R. [1] ; Galhardo, Rodrigo S. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: DNA Repair; v. 33, p. 78-89, SEP 2015.
Citações Web of Science: 5
Resumo

The SOS response is a universal bacterial regulon involved in the cellular response to DNA damage and other forms of stress. In Caulobacter crescentus, previous work has identified a plethora of genes that are part of the SOS regulon, but the biological roles of several of them remain to be determined. In this study, we report that two genes, hereafter named mmcA and mmcB, are involved in the defense against DNA damage caused by mitomycin C (MMC), but not against lesions induced by other common DNA damaging agents, such as UVC light, methyl methanesulfonate (MMS) and hydrogen peroxide. mmcA is a conserved gene that encodes a member of the glyoxalases/dioxygenases protein family, and acts independently of known DNA repair pathways. On the other hand, epistasis analysis showed that mmcB acts in the same pathway as imuC (dnaE2), and is required specifically for MMC-induced mutagenesis, but not for that induced by UV light, suggesting a role for MmcB in translesion synthesis-dependent repair of MMC damage. We show that the lack of MMC-induced mutability in the mmcB strain is not caused by lack of proper SOS induction of the imuABC operon, involved in translesion synthesis (TLS) in C. crescentus. Based on this data and on structural analysis of a close homolog, we propose that MmcB is an endonuclease which creates substrates for ImuABC-mediated TLS patches. (C) 2015 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 09/51387-7 - Regulação da mutagênese no modelo de Caulobacter crescentus e suas implicações para a evolução bacteriana
Beneficiário:Rodrigo da Silva Galhardo
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores