Gastroprotective activity of the hydroethanolic extract and isolated compounds from the leaves of Solanum cernuum Vell.

Autor(es)

Miranda, Mariza Abreu ^[1] ; Lemos, Marivane ^[1] ; Cowart, Kamila Alves ^[2] ; Rodenburg, Douglas ^[2] ; McChesney, James D. ^[2] ; Radwan, Mohamed M. ^[3] ; Jacometti Cardoso Furtado, Niege Aracari ^[1] ; Bastos, Jairo Kenupp ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Sao Paulo - Brazil

^[2] Ironstone Separat Inc, Oxford, MS 38655 - USA

^[3] Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod Res, University, MS 38677 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Ethnopharmacology; v. 172, p. 421-429, AUG 22 2015.
• Citações Web of Science: 10

Resumo

Ethnopharmacological relevance: Solanum cernuum yell. (Solanaceae) is a Brazilian medicinal plant, traditionally known as ``panaceia{''}. Its folk name is probably due to its wide range of applications in traditional medicine including the treatment of ulcers. Aim of the study: To evaluate the gastroprotective activities of the hydroethanolic extract (ESC) of S. cernuum and its major isolated compounds using in vivo gastric ulcer models. Material and methods: The ESC extract was obtained by maceration followed by percolation of the dried and powdered leaves of S. cernuum in ethanol:water (7:3). The major compounds in the extract were isolated by applying various preparative chromatographic techniques. The gastroprotective activity was evaluated in mice using different gastric ulcer-induced models. The anti-Helicobacter pylori activity was performed using the agar-well diffusion and broth microdilution methods. Results: The ESC extract showed gastroprotective effects in the assay of acute gastric ulcer-induced by HCl/EtOH, nonsteroidal anti-inflammatory drug, and acetic acid-induced chronic ulcer protocols. The results also demonstrated that the gastroprotection induced by ESC extract is related to the activity of nitric oxide and endogenous sulfhydryls, which are important gastroprotective factors. The ESC extract and the alkaloid cernumidine did not show activity against H. pylori in the concentrations tested. Conclusions: The present study showed that the crude extract of S. cernuum possessed gastroprotective activity which corroborating the traditional use of this plant for the treatment of gastric ulcers. The isolated flavonoids, quercitrin and afzelin as well as the phenylpropanoid, isoferulic acid are suggested to be the compounds responsible for the gastroprotective activity of S. cernuum extract. (C) 2015 Published by Elsevier Ireland Ltd. (AU)

• Processo FAPESP: 12/00715-7 - Solanum cernuum Vell: estudo fitoquímico, avaliação das atividades antiulcerogênica, antimicrobiana, citotóxica e obtenção de extrato seco por spray dryer
• Beneficiário: Mariza Abreu Miranda
• Modalidade de apoio: Bolsas no Brasil - Doutorado