Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems

Autor(es)

Apparicio, Maricy ^[1] ; Mostachio, Giuliano Q. ^[1] ; Motheo, Tathiana F. ^[1] ; Alves, Aracelle E. ^[1] ; Padilha, Luciana ^[1] ; Pires-Butler, Eliandra A. ^[1] ; Savi, Paula A. P. ^[1] ; Uscategui, Ricardo A. R. ^[1] ; Luvoni, Gaia C. ^[2] ; Vicente, Wilter R. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP - Brazil

^[2] Univ Milan, Dipartimento Sci Vet Salute Prod Anim & Sicurezza, I-20133 Milan - Italy

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 27, n. 7, p. 1082-1087, 2015.
• Citações Web of Science: 4

Resumo

The aim of this study was to evaluate the influence of different bi-phasic systems with gonadotrophins and steroids on in vitro maturation rates of oocytes obtained from bitches at different reproductive stages (follicular, luteal, anoestrous). In System A (control) oocytes were matured for 72h in base medium (BM) with 10IUmL(-1) human chorionic gonadotrophin (hCG), 1gmL(-1) progesterone (P4) and 1gmL(-1) oestradiol (E2); in bi-phasic System B oocytes were matured for 48h in BM with hCG and for 24h in BM with P4; in bi-phasic System C oocytes were matured for 48h in BM with hCG, P4 and E2, and for 24h in BM with P4; in System D, oocytes were cultured in BM without hormonal supplementation. Data were analysed by ANOVA. There was a positive effect of the bi-phasic systems on germinal vesicle breakdown, metaphase I and metaphase II rates, irrespective of reproductive status (P<0.05). Bi-phasic systems were also beneficial for cortical granule distribution (an indication of cytoplasmic maturation) and its relationship to nuclear status: 74.5% of the oocytes cultured in System B and 85.4% of those cultured in System C presented both nuclear and cytoplasmic maturation (P<0.001). The stage of the oestrous cycle did not influence maturation rates. (AU)

• Processo FAPESP: 06/51943-9 - Estudo da maturação nuclear e citoplasmática in vitro de oócitos de cadelas cultivados em meios sequenciais com hormônios e espermatozóides nas diferentes fases do ciclo estral
• Beneficiário: Maricy Apparício Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/50580-5 - Estudo da maturação nuclear e citoplasmática in vitro de oócitos de cadelas cultivados em meios sequenciais com hormônios e espermatozóides nas diferentes fases do ciclo estral
• Beneficiário: Wilter Ricardo Russiano Vicente
• Modalidade de apoio: Auxílio à Pesquisa - Regular