A simple protocol to determine lignin S/G ratio in plants by UHPLC-MS

Autor(es)

Mokochinski, Joao Benhur ^[1] ; Bataglion, Giovana Anceski ^[2] ; Kiyota, Eduardo ^[1] ; de Souza, Leticia Marrone ^[1] ; Mazzafera, Paulo ^[1] ; Helena Frankland Sawaya, Alexandra Christine ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Dept Plant Biol, Inst Biol, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 407, n. 23, p. 7221-7227, SEP 2015.
• Citações Web of Science: 6

Resumo

A simple extraction protocol and an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method for the determination of the syringyl/guaiacyl (S/G) ratio in lignin is reported herein. The method was entirely developed using stems of three Eucalyptus species, which were hydrolyzed with NaOH and partitioned with ethyl ether; vanillin (from the G monomer) and syringaldehyde (from S monomer) were quantified. The S/G ratios obtained were comparable to those usually reported for eucalyptus. The data for one of the eucalyptus species were compared with those obtained with a widely accepted method using thioacidolysis and gas chromatography-mass spectrometry (GC-MS). The method was also applied to sugarcane and showed to be reliable. The yield of the NaOH hydrolysis of the monolignols ranged from 89.94 to 95.69 %, with more than 77.12 % of recuperation in the liquid-liquid extraction. The whole analytical procedure was validated, achieving results with less than 4.38 % of variation. The lowest LOD and LOQ were 0.01 and 0.05 mu g/mL, respectively. In addition, the method combines reliability and a fast and direct quantification. (AU)

• Processo FAPESP: 11/51949-5 - Physiological responses of Eucalyptus globulus and E. grandis to high concentration of CO2 and temperature variations, identified by metabolomic and transcriptomic analyses
• Beneficiário: Paulo Mazzafera
• Linha de fomento: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE
• Processo FAPESP: 13/21306-0 - Desenvolvimento de métodos analíticos por uplc-ms para o estudo do metaboloma de duas espécies de eucalipto expostas a variações de temperatura e concentração de co2
• Beneficiário: João Benhur Mokochinski
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/21395-0 - Caracterização molecular da matéria orgânica sedimentar do Sistema Estuarino-Lagunar de Cananéia-Iguape por espectrometria de massas de ressonânica ciclotrônica de íons com transformada de Fourier
• Beneficiário: Giovana Anceski Bataglion
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
• Beneficiário: Paulo Mazzafera
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático